The Arabidopsis MALE STERILITY1 (MS1) gene is a transcriptional regulator of male gametogenesis, with homology to the PHD-finger family of transcription factors
The Arabidopsis MALE STERILITY1 (MS1) gene is a transcriptional regulator of male gametogenesis, with homology to the PHD-finger family of transcription factors
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DOI：
10.1046/j.1365-313x.2001.01125.x
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发表时间：
2001-10-01
期刊：
影响因子：
7.2
通讯作者：
Tighe, PJ
中科院分区：
文献类型：
--
作者：
Wilson, ZA;Morroll, SM;Tighe, PJ
我们在这里报告的拟南芥雄性不育基因，这是一个重要的孢子体控制因素花药和花粉发育的分子特征。纯合ms 1突变体不能产生有活力的花粉，但在其他方面表现正常。小孢子从四分体中释放出来后不久，花粉发生退化，此时绒毡层也出现异常的空泡化。MS 1基因在闭合花蕾的花药中低水平表达，在小孢子释放阶段的绒毡层中表达。在开放的花朵中看不到表情。推导的MS 1蛋白序列显示出很强的同源性PHD-指基序中发现的已知转录因子从人类，酵母和高等植物。已经确定了6个等位基因的MS 1，所有的结果在过早终止的MS 1蛋白质和损失的PHD-指基序。MS 1基因可能在雄配子发生和花药发育的特定阶段起着重要的转录调控作用。因此，MS 1为操纵高等植物雄性不育提供了有价值的工具。
We report here the molecular characterisation of the Arabidopsis MALE STERILITY1 gene, which is a critical sporophytic controlling factor for anther and pollen development. Homozygous ms1 mutants do not produce viable pollen, but are otherwise phenotypically normal. Degeneration of pollen occurs soon after microspore release from the tetrads, at which time the tapetum also appears abnormally vacuolated. The MS1 gene is expressed at low levels in anthers from closed buds, with expression in the tapetum at the stage of microspore release. No expression is seen in open flowers. The deduced MS1 protein sequence shows strong homology to the PHD-finger motif found in known transcription factors from humans, yeast and higher plants. Six alleles of ms1 have been identified; all result in premature termination of the MS1 protein and loss of the PHD-finger motif. MS1 is likely to play a key role in regulating transcription during specific stages of male gametogenesis and anther development. As such, MS1 provides a valuable tool for the manipulation of male sterility in higher plants.