The bovine papillomavirus E1 protein alters the host cell cycle and growth properties.
The bovine papillomavirus E1 protein alters the host cell cycle and growth properties.
复制标题
牛乳头瘤病毒 E1 蛋白改变宿主细胞周期和生长特性。
DOI：
10.1006/viro.1994.1517
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发表时间：
1994
期刊：
影响因子：
3.7
通讯作者：
Wilson,V
中科院分区：
文献类型：
--
作者：
Belyavskyi,M;Miller,J;Wilson,V
为了研究牛乳头瘤病毒E1蛋白对宿主细胞生长的影响，建立了一种稳定表达E1蛋白的细胞系CE 1。E1在CE 1细胞中的表达受地塞米松诱导型小鼠乳腺肿瘤病毒LTR启动子的控制。采用流式细胞术测定CE 1细胞内E1水平，同时测定细胞周期分布和细胞生长参数。CE 1细胞表达基础水平的E1，其在暴露于增加浓度的地塞米松后增加。随着E1表达的增加，观察到以下对细胞生长的影响：（1）总体细胞生成时间增加;（2）G1 + G 0期持续时间略微增加，而G2 + M期持续时间增加两倍。在表达E1的细胞中还观察到DNA含量大于G2期的细胞亚群的出现。去除地塞米松导致E1水平恢复到基础状态，同时恢复细胞生长特性和细胞周期时相分布。所有观察到的效应都是在没有其他乳头瘤病毒蛋白和乳头瘤病毒DNA复制的情况下观察到的。我们的结论是，E1是足以产生负面影响宿主细胞的生长，在G2 + M期观察到一个突出的效果。
A stable E1-expressing cell line, CE1, was developed to investigate the effect of bovine papillomavirus E1 protein on host cell growth. Expression of E1 in CE1 cells is under control of the dexamethasone-inducible mouse mammary tumor virus LTR promoter. Flow cytometry was used to determine the intracellular levels of E1 in CE1 cells in parallel with cell cycle distributions and cell growth parameters. CE1 cells expressed a basal level of E1 that was increased following exposure to increasing concentrations of dexamethasone. As E1 expression increased, the following effects on cell growth were observed: (1) the overall cell generation lime increased and (2) the duration of G1 + G0 phases increased slightly while the duration of G2 + M phases increased twofold. The appearance of a cell subpopulation with a greater than G2 phase DNA content was also observed in E1-expressing cells. Removal of dexamethasone resulted in a return of E1 levels to the basal state with a concomitant restoration of both cell growth properties and cell cycle phase distributions. All of the observed effects were seen in the absence of both other papillomaviral proteins and papillomaviral DNA replication. We conclude that E1 is sufficient to negatively affect host cell growth, with a prominent effect observed in the G2 + M phases.