Effects of FG7142 and immobilization stress on the gene expression in the neocortex of mice
Effects of FG7142 and immobilization stress on the gene expression in the neocortex of mice
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DOI：
10.1016/j.neures.2008.08.001
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发表时间：
2008-11-01
影响因子：
2.9
通讯作者：
Nishikawa, Toru
中科院分区：
文献类型：
--
作者：
Kurumaji, Akeo;Ito, Takashi;Nishikawa, Toru
Several psychiatric disorders are often precipitated or exacerbated by exposure to stressors. FG7142 (N-methyl-beta-carboline-3-carboxamide), a partial inverse agonist of benzodiazepine receptors, mimics the physiological (an increased release in the adrenal steroid hormone) and neurochemical (an enhanced neurotransmission of monoamines) changes induced by stressful stimuli.We examined the effects of FG7142 and immobilization stress on the gene expression of the mouse neocortex in order to obtain a new insight into the molecular stress-responsive system.The effect of FG7142 (20 mg/kg, i.p.) on the gene expression of the brain area was examined using a DNA microarray method. The genes showing a significant change in expression were investigated in further experiments using the quantitative RT-PCR method.There was an increase in the mRNA of seven genes in the neocortex of mice I h after treatment with FG7142. In addition, there was an increase in the mRNAs of five of the seven genes (Fos, Cyr61, Btg2, Adamts1, and Gem) in the neocortex of mice exposed to the stress for 1 h.The up-regulation of these five genes by both FG7142 and immobilization stress indicates that these genes may be involved in the stress-responsive system. Dysfunctions of the system may be associated with the pathophysiology of psychiatric disorders. (C) 2008 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
