Enhancement of natural cytotoxicity by beta-endorphin.
Enhancement of natural cytotoxicity by beta-endorphin.
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DOI：
10.4049/jimmunol.130.4.1658
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发表时间：
1983-04
影响因子：
4.4
通讯作者：
P. M. Mathews;C. Froelich;W. Sibbitt;A. Bankhurst
中科院分区：
文献类型：
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作者：
P. M. Mathews;C. Froelich;W. Sibbitt;A. Bankhurst
脑啡肽、β-内啡肽或神经系统产生的其他神经肽在免疫反应性改变中的作用一般尚不清楚。本研究旨在探讨这些神经肽对自然杀伤细胞(NK细胞)诱导的人自发性细胞毒的调节作用。用放射性标记的K562细胞用标准的~(51)Cr释放法测定其天然细胞毒性。β-内啡肽(30.5+/-11.5%，M+/-SE，相对增强50：1，效靶比(E：T)，10(-14)M-β-内啡肽)和甲硫氨酸-脑啡肽(甲硫氨酸-脑啡肽)(10(-9)M时相对增强27.4+/-9.7%)均显著增强NK细胞活性。相对增强的幅度与β-内啡肽浓度的增加显著相关。亮氨酸脑啡肽、α-内啡肽和吗啡不能增强NK活性。在所有受试E：T比值下，β-内啡肽对NK细胞活性的增强作用均增强。纳洛酮可抑制β-内啡肽和甲硫氨酸脑啡肽对NK细胞活性的增强作用。通过结合使用标准的51Cr释放和软琼脂糖单细胞分析，β-内啡肽增加了靶结合细胞中E：T细胞结合物的数量和活性杀伤细胞的数量。最大效应细胞回收能力提高了170%。这些研究为神经系统产生的神经肽改变免疫反应的机制提供了新的见解。
The role of enkephalins, beta-endorphin, or other neuropeptides produced by the nervous system in the alteration of immune responsiveness is generally unknown. The present studies were undertaken to investigate the role of these neuropeptides in the modulation of human spontaneous cytotoxicity induced by natural killer (NK) cells. Natural cytotoxicity was measured by using a standard 51Cr release assay with radiolabeled K562 cells. NK activity was significantly enhanced by both beta-endorphin (30.5 +/- 11.5%, M +/- SE, relative enhancement at 50:1, effector:target (E:T) ratio, 10(-14)M beta-endorphin) and methionine-enkephalin (met-enkephalin) (27.4 +/- 9.7% relative enhancement at 10(-9)M). The magnitude of relative enhancement significantly correlated with increasing concentrations of beta-endorphin. Leucine-enkephalin, alpha-endorphin, and morphine did not augment NK activity. The enhancement of NK activity with beta-endorphin increased at all E:T ratios tested. Naloxone inhibited the augmentation of NK activity produced by beta-endorphin and met-enkephalin. By using a combination of a standard 51Cr release and soft agarose single cell analysis assays, beta-endorphin increased both the number of E:T cell conjugates and the number of active killer cells among target-binding cells. The maximal effector cell recycling capacity was increased by 170%. These studies provide new insight into the mechanisms by which neuropeptides produced by the nervous system can alter immune responsiveness.