An AIEgen-Peptide Conjugate as a Phototheranostic Agent for Phagosome-Entrapped Bacteria
An AIEgen-Peptide Conjugate as a Phototheranostic Agent for Phagosome-Entrapped Bacteria
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DOI：
10.1002/anie.201906099
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发表时间：
2019-09-30
影响因子：
16.6
通讯作者：
Liu, Bin
中科院分区：
文献类型：
--
作者：
Qi, Guobin;Hu, Fang;Liu, Bin
细胞内细菌的检测和消除仍然是一个重大挑战。在这项工作中，我们报告了一种聚集诱导发射（AIE）生物探针，它可以通过动态过程检测细菌感染并杀死巨噬细胞内存活的细菌，特别是通过受感染的巨噬细胞中的caspase-1激活以及残留物在含有细菌的吞噬体上的积累来对探针进行特定的分子剪裁，从而导致发光的荧光信号。此外，AIEgen可以作为光敏剂来产生活性氧（ROS）；细菌吞噬体中单位面积的平均ROS指示剂荧光信号强度比细胞质中高约2.7倍。这反过来又诱导细菌高效杀灭，并且对巨噬细胞的细胞毒性最小。我们设想这种特定的发光生物探针可能为选择性、灵敏地检测和根除细胞内细菌感染提供一种新方法。
The detection and elimination of intracellular bacteria remain a major challenge. In this work, we report an aggregation-induced emission (AIE) bioprobe that can detect bacterial infection and kill bacteria surviving inside macrophages through a dynamic process, notably specific molecular tailoring of the probe by caspase-1 activation in infected macrophages and accumulation of the residue on phagosomes containing bacteria, leading to light-up fluorescent signals. Moreover, the AIEgen can serve as a photosensitizer for generation of reactive oxygen species (ROS); and the average ROS indicator fluorescent signal intensity per unit area in the bacterial phagosomes is approximately 2.7-fold higher than that in the cytoplasm. This, in turn, induces bacteria killing with high efficiency and minimal cytotoxicity towards macrophages. We envision that this specific light-up bioprobe may provide a new approach for selective and sensitive detection and eradication of intracellular bacterial infections.