Laser photoacoustic trace gas detection, an extremely sensitive technique applied in biological research
Laser photoacoustic trace gas detection, an extremely sensitive technique applied in biological research
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DOI：
10.1080/10739149808002690
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发表时间：
1998-01-01
影响因子：
1.6
通讯作者：
Parker, DH
中科院分区：
文献类型：
--
作者：
Hekkert, ST;Staal, MJ;Parker, DH
结合两个实例，论述了红外激光光声微量气体检测设备在生物研究中的应用。采用基于CO2激光的光声微量气体检测系统，在1分钟的时间尺度上跟踪蓝藻固氮过程的时间依赖模式。由于乙烯检测系统的高灵敏度（检测限6万亿分之一；6.10(12)），固定过程可以在流式系统中在线跟踪。经过50 h的黑暗孵育后，细菌在一定的光照时间后才开始固氮，这表明细菌缺乏启动固氮所需的碳水化合物。
The use of infrared laser based photo-acoustic trace gas detection equipment in biological research is discussed on the basis bf two examples. A CO2 laser based photo-acoustic trace gas detection system is employed to follow the time-dependent pattern of the nitrogen fixation process by the cyanobacteria Nodularia Spumigena on a one-minute time scale. Due to the high sensitivity of the detection system for ethylene (detection limit 6 part per trillion; 6.10(12)), the fixation process can be followed on-line in a flow-through system. Following a 50 h dark incubation period, the bacteria show nitrogen fixation only after a certain illumination period, indicating lack of carbohydrates needed to start the nitrogen fixation.