VIGR - a novel inducible adhesion family G-protein coupled receptor in endothelial cells
VIGR - a novel inducible adhesion family G-protein coupled receptor in endothelial cells
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DOI：
10.1016/j.febslet.2004.05.038
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发表时间：
2004-07-02
期刊：
影响因子：
3.5
通讯作者：
Lipp, J
中科院分区：
文献类型：
--
作者：
Stehlik, C;Kroismayr, R;Lipp, J
利用信号序列捕捉技术筛选差异表达的分泌型和膜型蛋白，我们鉴定了G蛋白偶联受体(GPCRs)黏附家族中的一个新成员，称为血管诱导型GPCRVIGR。VIGR含有C1r-C1s、Uegf和Bmp1(Cub)和五角蛋白(Ptx)样模块和粘蛋白样间隔区，随后是7个跨膜区。通过表面生物素化和免疫荧光分析，我们证明了内源性的高度糖基化的VIGR在内毒素或凝血酶处理的内皮细胞(ECs)的细胞表面表达，并且诱导表达是由MAP激酶介导的，而不是由核因子-kappaB介导的。我们发现VIGR选择性地表达于来自较大血管的内皮细胞，而不是来自微血管的内皮细胞。综上所述，VIGR代表了黏附家族中的一个新的GPCR，其独特之处在于它的长胞外结构域由CUB和PTX样模块组成，并且它可以被内毒素和凝血酶诱导，提示它在细胞黏附中发挥重要作用，并可能将炎症和凝血联系起来。(C)2004年欧洲生化学会联合会。爱思唯尔出版，版权所有。
Using a signal sequence trap for selection of differentially expressed secretory and membrane proteins, we identified a novel member of the adhesion family of G-protein coupled receptors (GPCRs), termed vascular inducible GPCR (VIGR). VIGR contains C1r-C1s, Uegf and Bmp1 (CUB) and pentraxin (PTX)-like modules and a mucin-like spacer, followed by seven transmembrane domains. By surface biotinylation as well as by immunofluorescence analysis we demonstrate that endogenous, highly glycosylated VIGR is expressed on the cell surface of endothelial cells (ECs) upon LPS or thrombin treatment, and inducible expression is mediated by MAP kinases, but not NF-kappaB. We show that VIGR is selectively expressed in ECs derived from larger vessels, but not from microvessels. In summary, VIGR represents a novel GPCR of the adhesion family, which is unique in its long extra-cellular domain comprising CUB and PTX-like modules and in its inducibility by LPS and thrombin in a subset of ECs, suggesting an important function in cell-adhesion and potentially links inflammation and coagulation. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.