THE EFFECT OF HYPOTHERMIA ON TRANSIENT MIDDLE CEREBRAL-ARTERY OCCLUSION IN THE RAT
THE EFFECT OF HYPOTHERMIA ON TRANSIENT MIDDLE CEREBRAL-ARTERY OCCLUSION IN THE RAT
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DOI：
10.1038/jcbfm.1992.86
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发表时间：
1992-07-01
影响因子：
6.3
通讯作者：
GARCIA, JH
中科院分区：
文献类型：
--
作者：
CHEN, H;CHOPP, M;GARCIA, JH
我们研究了中度全身体温过低（30度C）对大鼠瞬时脑动脉闭塞（MCAO）的影响。雄性Wistar大鼠通过将缝合线插入内部颈动脉的腔内并阻塞MCA的起源，从而遭受2小时的缺血。实验组是（a）MCAO在37度C体温下诱导的（n = 15）； （b）在缺血之前诱导的30度体温度，并维持MCAO 2小时，再灌注1小时（n = 12）； （c）MCAO具有在正常热（n = 3）和低温MCAO大鼠（n = 2）中测得的区域脑和体温。再灌注后96小时进行组织病理学评估。所有常规的MCAO动物均表现出缺血性梗死，涉及副粒细胞，巨噬细胞和微血管增殖的同侧皮层和基底神经节。低温MCAO动物表现出轻微的缺血性损伤，从选择性神经元损伤到梗塞的小焦点区域，炎症反应最小。我们的数据表明，瞬时缺血是通过使用内部缝合方法阻塞MCA会导致可重复的脑部病变的，并且中等低温对瞬态MCAO后的脑损伤具有深远的保护作用。
We investigated the effect of moderate whole body hypothermia (30-degrees-C) on transient middle cerebral artery occlusion (MCAO) in the rat. Male Wistar rats were subjected to 2 h of ischemia by inserting a suture into the lumen of the internal carotid artery and occluding the origin of the MCA. Experimental groups were (a) MCAO induced at 37-degrees-C body temperature (n = 15); (b) 30-degrees-C body temperature induced prior to ischemia and maintained for 2 h of MCAO and 1 h of reperfusion (n = 12); and (c) MCAO with regional brain and body temperatures measured in normothermic (n = 3) and hypothermic MCAO rats (n = 2). Histopathological evaluation was performed 96 h after reperfusion. All normothermic MCAO animals exhibited ischemic infarct involving the ipsilateral cortex and basal ganglia with infiltration of neutrophils, macrophages, and microvascular proliferation. Hypothermic MCAO animals exhibited minor ischemic damage ranging from selective neuronal injury to small focal areas of infarct with minimal inflammatory response. Our data demonstrate that transient ischemia induced by using the intraarterial suture method to occlude the MCA results in a reproducible brain lesion and that moderate hypothermia has a profound protective effect on the brain injury after transient MCAO.