SEVERE FOLATE-DEFICIENCY CAUSES SECONDARY DEPLETION OF CHOLINE AND PHOSPHOCHOLINE IN RAT-LIVER
SEVERE FOLATE-DEFICIENCY CAUSES SECONDARY DEPLETION OF CHOLINE AND PHOSPHOCHOLINE IN RAT-LIVER
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DOI：
10.1093/jn/124.11.2197
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发表时间：
1994-11-01
影响因子：
4.2
通讯作者：
MASON, JB
中科院分区：
文献类型：
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作者：
KIM, YI;MILLER, JW;MASON, JB
此前已有研究表明，胆碱缺乏会导致大鼠肝叶酸浓度降低。进行了两个独立的实验来研究相反的现象：叶酸缺乏是否会导致肝胆碱消耗。在实验1中，通过喂食含有（每公斤饲料）1.4g胆碱、0mg叶酸和10g琥珀酰磺胺噻唑的氨基酸限定饮食来诱导大鼠严重叶酸缺乏。对照大鼠喂食含有 8 mg 叶酸/kg 的相同饮食。 4周后，与对照组相比，严重叶酸缺乏大鼠的血浆和肝脏叶酸浓度显着降低（P < 0.001），肝胆碱和磷酸胆碱浓度分别降低65%和80%（P < 0.001）。在实验2中，通过饲喂与上述相同的饮食但省略琥珀酰磺胺噻唑来诱导大鼠中度叶酸缺乏。 24周后，与对照组相比，中度叶酸缺乏大鼠出现显着的全身叶酸缺乏（P < 0.001）。与对照组相比，中度叶酸缺乏大鼠的肝胆碱浓度适度降低（36%，P = 0.087）。两组之间的肝脏磷酸胆碱浓度没有检测到显着差异。这些结果表明，严重的叶酸缺乏会导致大鼠继发性肝胆碱缺乏。
It has previously been shown that choline deficiency causes depletion of hepatic folate concentration in rats. Two separate experiments were undertaken to investigate the converse phenomenon: whether folate deficiency would lead to depletion of hepatic choline. In Experiment 1, severe folate deficiency was induced in rats by feeding an amino acid-defined diet containing (per kg diet) 1.4 g choline, 0 mg folate and 10 g succinylsulfathiazole. Control rats were fed the same diet containing 8 mg folate/kg. After 4 wk, plasma and hepatic folate concentrations were significantly depleted in the severely folate-deficient rats compared with controls (P < 0.001), and hepatic choline and phosphocholine concentrations were 65 and 80% lower, respectively (P < 0.001). In Experiment 2, moderate folate deficiency was induced in rats by feeding the same diet as described above, but with the succinylsulfathiazole omitted. After 24 wk, significant systemic folate deficiency was present in the moderately folate-deficient rats compared with controls (P < 0.001). A modest reduction (36%, P = 0.087) in hepatic choline concentration was observed in the moderately folate-deficient rats compared with controls. No significant differences in hepatic phosphocholine concentrations were detected between the two groups. These results indicate that severe folate deficiency causes secondary hepatic choline deficiency in rats.