Conducting Polymer-Based Electrochemical Aptasensor for the Detection of Adenosine
Conducting Polymer-Based Electrochemical Aptasensor for the Detection of Adenosine
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DOI：
10.1021/acsapm.1c01348
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发表时间：
2021-11
影响因子：
5
通讯作者：
Damilola O. Runsewe;Grace Haya;Tania Betancourt;J. Irvin
中科院分区：
文献类型：
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作者：
Damilola O. Runsewe;Grace Haya;Tania Betancourt;J. Irvin
新兴的研究领域的导电聚合物为基础的电化学生物传感器已经揭示了需要开发的技术，可以使容易的功能化与生物识别分子，并提高生物传感器的稳定性。本工作利用导电共聚物作为转导剂，研制了一种检测小分子腺苷的电化学生物传感器。首先，开发了一种方法来改性氧化铟锡涂覆的载玻片的表面，以实现稳健的共聚物沉积。然后在改性载玻片的表面上电化学生长3，4-亚乙基二氧噻吩（EDOT）和2 H-噻吩并[3，4-B][1，4]二氧杂环庚三烯-3，3（4 H）-二乙酸（ProDOT-（COOH）2）共聚物。该共聚物用于将对腺苷特异性的适体共价连接到生物传感平台，以提供具有靶选择性的系统。研究了导电聚合物在适体附着之前和之后在电解质水溶液中的电活性。使用荧光显微镜和循环伏安法确认适体与导电聚合物的附着。将所制备的核酸适体传感器用于腺苷的电化学检测，并对传感器的性能进行了研究。腺苷适体传感器的检测限为2.33 nM，线性范围为9.6 nM至600 μM。腺苷适体传感器显示出良好的选择性对竞争性干扰剂和特异性相对于乱序寡核苷酸立场。此外，传感器在0.1 M磷酸盐缓冲盐水或氩气中储存长达6天时表现出良好的稳定性。
Emerging research in the area of conducting polymer-based electrochemical biosensors has revealed the need for the development of techniques that can enable easy functionalization with biorecognition molecules and enhance biosensor stability. In this work, an electrochemical biosensor for the detection of the small molecule adenosine was developed utilizing a conducting copolymer as a transducing agent. First, a method was developed to modify the surface of indium tin oxide-coated glass slides to enable robust copolymer deposition. A 3,4-ethylenedioxythiophene (EDOT) and 2H-thieno[3,4-b][1,4]dioxepin-3,3(4H)-diacetic acid (ProDOT–(COOH)2) copolymer was then electrochemically grown on the surface of the modified slides. This copolymer was used to covalently attach an aptamer specific to adenosine to the biosensing platform to provide the system with target selectivity. The electroactivity of the conducting polymer before and after aptamer attachment in aqueous electrolyte solutions was studied. The attachment of the aptamers to the conducting polymer was confirmed using fluorescence microscopy and cyclic voltammetry. The fabricated aptamer-based sensors were then used for the electrochemical detection of adenosine, and the performance of the sensor was investigated. The adenosine aptasensor had a limit of detection of 2.33 nM and a linear range from 9.6 nM to 600 μM. The adenosine aptasensor showed good selectivity against competing interfering agents and specificity relative to scrambled oligonucleotide stands. In addition, the sensor showed good stability for up to 6 days when stored in 0.1 M phosphate-buffered saline or argon.