High-resolution confocal microscopy by saturated excitation of fluorescence
High-resolution confocal microscopy by saturated excitation of fluorescence
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DOI：
10.1103/physrevlett.99.228105
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发表时间：
2007-11-30
影响因子：
8.6
通讯作者：
Kawata, Satoshi
中科院分区：
文献类型：
--
作者：
Fujita, Katsumasa;Kobayashi, Minoru;Kawata, Satoshi
我们证明了使用饱和激发共聚焦荧光显微镜，以提高空间分辨率。在所提出的技术中，我们在时间上调制的激发强度和检测的谐波调制的荧光信号，这是由饱和激发在激光焦点的中心。理论和实验研究表明，解调的荧光信号是非线性的激发强度成正比，并有助于提高在三维空间分辨率超过光的衍射极限。
We demonstrate the use of saturated excitation in confocal fluorescence microscopy to improve the spatial resolution. In the proposed technique, we modulate the excitation intensity temporally and detect the harmonic modulation of the fluorescence signal which is caused by the saturated excitation in the center of the laser focus. Theoretical and experimental investigations show that the demodulated fluorescence signal is nonlinearly proportional to the excitation intensity and contributes to improve the spatial resolution in three dimensions beyond the diffraction limit of light.