Yeast Sm-like proteins function in mRNA decapping and decay
Yeast Sm-like proteins function in mRNA decapping and decay
复制标题
DOI：
10.1038/35006676
复制
发表时间：
2000-03-30
期刊：
影响因子：
64.8
通讯作者：
Parker, R
中科院分区：
文献类型：
--
作者：
Tharun, S;He, WH;Parker, R
真核生物中信使RNA降解的主要机制之一是通过去腺苷化依赖性去帽发生，其导致5 '至3'衰变（1，2）。已经鉴定了一个Sm样（Lsm）蛋白家族，其成员含有“Sm”序列基序，与U6小核RNA形成复合物，并且是前mRNA剪接所需的（3-9）。在这里，我们表明，在七个酵母Lsm蛋白（Lsm 1-Lsm 7）的突变也导致抑制mRNA脱帽。此外，Lsm 1-Lsm 7蛋白与mRNA去帽酶（Dcp 1）、去帽激活剂（Pat 1/Mrt 1）和mRNA共免疫沉淀。这表明Lsm蛋白可能通过与mRNA和去帽机制的相互作用来促进去帽。此外，在mRNA衰变中起作用的Lsm复合物似乎与U6相关的Lsm复合物不同，表明Lsm蛋白形成影响mRNA代谢不同方面的特定复合物。
One of the main mechanisms of messenger RNA degradation in eukaryotes occurs by deadenylation-dependent decapping which leads to 5'-to-3' decay(1,2). A family of Sm-like (Lsm) proteins has been identified, members of which contain the 'Sm' sequence motif, form a complex with U6 small nuclear RNA and are required for pre-mRNA splicing(3-9). Here we show that mutations in seven yeast Lsm proteins (Lsm1-Lsm7) also lead to inhibition of mRNA decapping. In addition, the Lsm1-Lsm7 proteins coimmunoprecipitate with the mRNA decapping enzyme (Dcp1), a decapping activator (Pat1/Mrt1) and with mRNA. This indicates that the Lsm proteins may promote decapping by interactions with the mRNA and the decapping machinery. In addition, the Lsm complex that functions in mRNA decay appears to be distinct from the U6-associated Lsm complex, indicating that Lsm proteins form specific complexes that affect different aspects of mRNA metabolism.