Pathogenesis of leptospirosis:: interaction of Leptospira interrogans with in vitro cultured mammalian cells
Pathogenesis of leptospirosis:: interaction of Leptospira interrogans with in vitro cultured mammalian cells
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DOI：
10.1007/s00430-007-0047-0
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发表时间：
2007-12-01
影响因子：
5.4
通讯作者：
Yan, Jie
中科院分区：
文献类型：
--
作者：
Liu, Yunying;Zheng, Wei;Yan, Jie
通过一系列形态学分析研究了强毒钩端螺旋体与小鼠单核巨噬细胞样J774A.1细胞和非洲绿色猴肾成纤维细胞从粘附到内化的相互作用。丰塔纳银染色显示，只有致病性钩端螺旋体能够附着到宿主细胞，并且附着模式取决于它们与之相互作用的细胞类型。透射电子显微镜（TEM）分析证实了形成的钩端螺旋体的吞噬体和共聚焦显微镜分析证实了它们与溶酶体在巨噬细胞中的共定位。F-actin重排检测结果表明，强毒钩端螺旋体通过非依赖于微生物的途径侵入宿主细胞。
Interactions of virulent Leptospira interrogans with murine monocyte-macrophage-like J774A.1 cells and Vero (African green monkey kidney fibroblasts) cells from attachment to internalization were investigated by a series of morphological analysis. Fontana silver staining revealed that only the pathogenic leptospires were able to attach to host cells and the attachment pattern varied depending on cell types that they interacted with. Transmission electron microscopy (TEM) analysis confirmed the formation of the leptospires-containing phagosomes and their colocalization with lysosomes in macrophages were verified by confocal microscopic analysis. Results of F-actin rearrangements examination indicated that virulent leptospires invaded host cells via a microfilament-independent pathway.