Membrane-anchored cyclin A2 triggers Cdc2 activation in Xenopus oocyte
Membrane-anchored cyclin A2 triggers Cdc2 activation in Xenopus oocyte
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DOI：
10.1016/s0014-5793(01)02920-9
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发表时间：
2001-10-12
期刊：
影响因子：
3.5
通讯作者：
Jessus, C
中科院分区：
文献类型：
--
作者：
Faivre, J;Frank-Vaillant, M;Jessus, C
在非洲爪蟾卵母细胞中，新合成的细胞周期蛋白和Cdc 2之间形成的复合物有助于Cdc 2激酶激活，从而触发减数分裂。有人提出细胞质膜可能参与这一过程。为了研究这种可能性，我们在卵母细胞中注射了两个不可降解的人类细胞周期蛋白A2突变体锚定到内质网（ER）膜。它们编码截短的细胞周期蛋白A2-Delta 152和病毒或细胞ER靶向结构域之间的融合蛋白。我们发现，这两个突变体是完全功能的有丝分裂细胞周期蛋白在非洲爪蟾卵母细胞中表达时，结合Cdc 2和激活M期促进因子。(C)2001年由Elsevier Science B. V.代表欧洲生物化学学会联合会出版。
In Xenopus oocyte, the formation of complexes between neosynthesized cyclins and Cdc2 contributes to Cdc2 kinase activation that triggers meiotic divisions. It has been proposed that cytoplasmic membranes could be involved in this process. To investigate this possibility, we have injected in the oocyte two undegradable human cyclin A2 mutants anchored to the endoplasmic reticulum (ER) membrane. They encode fusion proteins between the truncated cyclin A2-Delta 152 and a viral or cellular ER-targeting domain. We show that both mutants are fully functional as mitotic cyclins when expressed in Xenopus oocytes, bind Cdc2 and activate M-phase promoting factor. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.