Polymorphism in abalone fertilization proteins is consistent with the neutral evolution of the egg's receptor for lysin (VERL) and positive Darwinian selection of sperm lysin
Polymorphism in abalone fertilization proteins is consistent with the neutral evolution of the egg's receptor for lysin (VERL) and positive Darwinian selection of sperm lysin
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DOI：
10.1093/oxfordjournals.molbev.a003813
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发表时间：
2001-03-01
影响因子：
10.7
通讯作者：
Vacquier, VD
中科院分区：
文献类型：
--
作者：
Swanson, WJ;Aquadro, CF;Vacquier, VD
自由产卵的海洋无脊椎动物物种特异性受精的进化对生殖隔离很重要，并可能有助于物种形成。鲍鱼（balbali genus Haliotis）中介导物种特异性受精的蛋白质的生物化学和进化已被广泛研究。非酶精子蛋白溶酶通过物种特异性结合其卵子受体VERL在卵黄膜上制造一个洞。正达尔文选择促进了溶酶的分化。相比之下，VERL的演变并没有偏离中立性。在此，我们克隆了一个新的VERL非重复区域，并对红色（Haliotis rufescens）和粉红色（Haliotis瓦罗塔）鲍鱼进行了种内多态性调查，以探索影响VERL的进化过程。六项统计检验表明，VERL的进化没有偏离中性。有趣的是，在粉鲍鱼的VERL序列中有一个细分，并且群体之间缺乏杂合个体，这表明分类交配的进化可能正在进行中。这些结果与假设卵子VERL是中性进化的模型一致，可能是由于其重复结构，而精子溶酶则受到积极的达尔文选择，以在精子竞争中保持两种蛋白质的有效相互作用。
The evolution of species-specific fertilization in free-spawning marine invertebrates is important for reproductive isolation and may contribute to speciation. The biochemistry and evolution of proteins mediating species-specific fertilization have been extensively studied in the abalone (genus Haliotis). The nonenzymatic sperm protein lysin creates a hole in the egg vitelline envelope by species-specifically binding to its egg receptor VERL. The divergence of lysin is promoted by positive Darwinian selection. In contrast, the evolution of VERL does not depart from neutrality. Here, we cloned a novel nonrepetitive region of VERL and performed an intraspecific polymorphism survey for red (Haliotis rufescens) and pink (Haliotis corrugata) abalones to explore the evolutionary farces affecting VERL. Six statistical tests showed that the evolution of VERL did not depart from neutrality. Interestingly, there was a subdivision in the VERL sequences in the pink abalone and a lack of heterozygous individuals between groups, suggesting that the evolution of assortative mating may be in progress. These results are consistent with a model which posits that egg VERL is neutrally evolving, perhaps due to its repetitive structure, while sperm lysin is subjected to positive Darwinian selection to maintain efficient interaction of the two proteins during sperm competition.