Purine levels in the intact rat brain. Studies with an implanted perfused hollow fibre
Purine levels in the intact rat brain. Studies with an implanted perfused hollow fibre
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完整大鼠大脑中的嘌呤水平。
DOI：
10.1016/0304-3940(82)90338-x
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发表时间：
1982
影响因子：
2.5
通讯作者：
B. Fredholm
中科院分区：
文献类型：
--
作者：
T. Zetterström;L. Vernet;U. Ungerstedt;U. Tossman;B. Jonzon;B. Fredholm
在氟烷麻醉下，将一根细透析管立体定向植入SD大鼠尾状核，灌注林格氏液，流速为2 μl/min，开始时嘌呤流出率很高，但灌注1-2 h后，嘌呤流出率基本不变（麻醉-腺苷0.4 ± 0.04μM，肌苷0.8 ± 0.2μM;非麻醉-腺苷0.33 ± 0.03μM，肌苷0.21 ± 0.07μM）。缺氧（9%O2）使水平增加3倍以上。腺苷脱氨酶抑制剂EHNA增加腺苷水平，降低肌苷水平。腺苷的体外回收率约为30%。因此，我们得出结论，大鼠脑中腺苷的自由交换浓度可能为1-2 μM。这一水平足以影响中枢神经功能。
A thin dialysis tube was implanted stereotaxically under halothane anesthesia in the caudate nucleus of Sprague-Dawley rats and perfused with Ringer solution at a rate of 2 μl/min. Initially there was a high rate of purine outflow but after 1–2 h of perfusion the rate was essentially constant (anesthetized - adenosine 0.4 ± 0.04μM, inosine 0.8 ± 0.2μM; non-anesthetized - adenosine 0.33 ± 0.03μM, inosine 0.21 ± 0.07μM). Hypoxia (9% O2) increased the levels more than 3-fold. The adenosine deaminase inhibitor erythro-2-(2-hydroxy-3-nonyl)adenine (EHNA) increased the adenosine level and decreased the inosine level. In vitro recovery of adenosine was about 30%. Therefore, we conclude that the free exchangable concentration of adenosine in the rat brain is likely to be 1–2 μM. This level is high enough to potentially affect central nervous function.