Adam12 and Inc015192 act as ceRNAs in breast cancer by regulating miR-34a
Adam12 and Inc015192 act as ceRNAs in breast cancer by regulating miR-34a
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Adam12 和 lnc015192 通过调节 miR-34a 在乳腺癌中充当 ceRNA。
DOI：
10.1038/s41388-018-0410-1
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发表时间：
2018-12-06
期刊：
影响因子：
8
通讯作者：
Xie, Xiaoming
中科院分区：
文献类型：
--
作者：
Huang, Xiaojia;Xie, Xinhua;Xie, Xiaoming
据报道，长链非编码rna （lncRNAs）在多种癌症的进展中起着至关重要的作用。然而，lncrna在乳腺癌中的功能仍有待发现。我们使用微阵列技术来鉴定miR-34a敲除或未敲除的乳腺组织中差异表达的mrna和lncrna。为了探究差异表达的mRNA和lncRNA在乳腺癌中的功能，我们进行了一系列实验。我们发现，Adam12和lnc015192在miR-34a敲除的乳腺组织中显著上调。下调Adam12和lnc015192抑制乳腺癌细胞迁移、侵袭和上皮间质转化（EMT）。进一步的实验表明，lnc015192通过作为miR-34a的竞争内源性RNA （ceRNA）调节Adam12的表达。综上所述，我们的研究表明Adam12和lnc015192通过ceRNA机制部分通过海绵作用miR-34a促进乳腺癌转移。
Long non-coding RNAs (lncRNAs) are reported to play vital roles in the progress of multiple cancers. However, the functions of lncRNAs in breast cancer remain to be discovered. We performed microarrays to identify the differentially expressed mRNAs and lncRNAs in breast tissues with or without miR-34a knockout. To explore the functions of the differentially expressed mRNA and lncRNA in breast cancer, we conducted a series of experiments. We found that Adam12 and lnc015192 were significantly upregulated in miR-34a knockout breast tissues. Knockdown of Adam12 and lnc015192 inhibited breast cancer cell migration, invasion, and epithelial-mesenchymal transition (EMT). Further experiments revealed that lnc015192 regulated Adam12 expression by functioning as a competing endogenous RNA (ceRNA) for miR-34a. In summary, our study demonstrate that Adam12 and lnc015192 promote breast cancer metastasis partly by sponging miR-34a through the ceRNA mechanism.