Cloning and partial nucleotide sequence of human immunoglobulin mu chain cDNA from B cells and mouse-human hybridomas.
Cloning and partial nucleotide sequence of human immunoglobulin mu chain cDNA from B cells and mouse-human hybridomas.
复制标题
来自 B 细胞和小鼠-人杂交瘤的人免疫球蛋白 mu 链 cDNA 的克隆和部分核苷酸序列。
DOI：
10.1073/pnas.77.10.6027
复制
发表时间：
1980
影响因子：
11.1
通讯作者：
Croce,CM
中科院分区：
文献类型：
--
作者：
Dolby,TW;Devuono,J;Croce,CM
编码 mu 和 kappa 人免疫球蛋白多肽的纯化 mRNA 在体外进行翻译，并对其产物进行表征。 mu 特异性 mRNA 来自人类淋巴母细胞 (GM607) 和分泌人 mu 链的小鼠-人体细胞杂交体 (α D5-H11-BC11)，被复制到 cDNA 中并插入质粒 pBR322 中。通过与标记探针的集落杂交、质粒选择的 mu mRNA 的体外翻译和 DNA 核苷酸序列测定相结合来鉴定获得的几种重组 cDNA。一种重组 DNA（其序列已部分确定）包含从 C3 恒定区结构域到羧基末端片段（总共 155 个氨基酸）的部分密码子，以及直至人 mu mRNA 的 Poly(A) 位点的整个 3' 非编码序列。序列 A-A-U-A-A 出现在人 mu mRNA 中的 Poly(A) 添加位点之前 12 个核苷酸处。在小鼠和人类 mu mRNA 3' 编码和非编码序列中观察到相当大的序列同源性。
Purified mRNAs coding for mu and kappa human immunoglobulin polypeptides were translated in vitro and their products were characterized. The mu-specific mRNAs, derived from both human lymphoblastoid cells (GM607) and from a mouse-human somatic cell hybrid secreting human mu chains (alpha D5-H11-BC11), were copied into cDNAs and inserted into the plasmid pBR322. Several recombinant cDNAs that were obtained were identified by a combination of colony hybridization with labeled probes, in vitro translation of plasmid-selected mu mRNAs, and DNA nucleotide sequence determination. One recombinant DNA, for which the sequence has been partially determined, contains the codons for part of the C3 constant region domain through the carboxy-terminal piece (155 amino acids total) as well as the entire 3' noncoding sequence up to the poly(A) site of the human mu mRNA. The sequence A-A-U-A-A occurs 12 nucleotides prior to the poly(A) addition site in the human mu mRNA. Considerable sequence homology is observed in the mouse and human mu mRNA 3' coding and noncoding sequences.