Antigenic and genetic analyses of H1N1 influenza A viruses from European pigs
Antigenic and genetic analyses of H1N1 influenza A viruses from European pigs
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DOI：
10.1099/0022-1317-78-3-553
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发表时间：
1997-03-01
影响因子：
3.8
通讯作者：
Alexander, DJ
中科院分区：
文献类型：
--
作者：
Brown, IH;Ludwig, S;Alexander, DJ
对1981年以来从欧洲猪群中分离的H1N1甲型流感病毒进行了抗原性和遗传学检查，并与其他来源的H1N1病毒进行了比较。来自猪和鸟类的H1N1病毒可分为三组：禽流感病毒、经典猪流感病毒和“禽流感样”猪流感病毒。在HI试验中，“禽样”猪病毒与抗经典猪病毒的单克隆抗体的反应性较低或无反应性与血凝素（HA）抗原位点内的氨基酸取代有关。HA基因的系统发育分析显示，来自欧洲猪的经典猪病毒彼此最相似，并且与北美猪株密切相关，而“禽样”猪病毒与禽病毒聚类。1992年在英国引入猪的“禽样”病毒显然直接来源于当时欧洲大陆猪的毒株。与人类H1N1病毒相比，所检测的猪病毒的HA基因在抗原位点上经历了有限的变异，并且还包含更少的潜在糖基化位点。HA表现出抗原漂移，这是更显着的“禽样”猪病毒比经典的猪株。对两种最近的“类禽”猪病毒的遗传分析表明，所有的RNA片段与A型禽流感病毒的RNA片段最接近。
H1N1 influenza A viruses isolated from pigs in Europe since 1981 were examined both antigenically and genetically and compared with H1N1 viruses from other sources. H1N1 viruses from pigs and birds could be divided into three groups: avian, classical swine and 'avian-like' swine viruses. Low or no reactivity of 'avian-like' swine viruses in HI tests with monoclonal antibodies raised against classical swine viruses was associated with amino acid substitutions within antigenic sites of the haemagglutinin (HA). Phylogenetic analysis of the HA gene revealed that classical swine viruses from European pigs are most similar to each other and are closely related to North American swine strains, whilst the 'avian-like' swine viruses cluster with avian viruses. 'Avian-like' viruses introduced into pigs in the UK in 1992 apparently originated directly from strains in pigs in continental Europe at that time. The HA genes of the swine viruses examined had undergone limited variation in antigenic sites and also contained fewer potential glycosylation sites compared to human H1N1 viruses. The HA exhibited antigenic drift which was more marked in 'avian-like' swine viruses than in classical swine strains. Genetic analyses of two recent 'avian-like' swine viruses indicated that all the RNA segments are related most closely to those of avian influenza A viruses.