ENZYME HISTOCHEMICAL-CHARACTERISTICS OF HUMAN AND KITTEN ODONTOCLASTS AND KITTEN OSTEOCLASTS - COMPARATIVE-STUDY USING WHOLE CELLS
ENZYME HISTOCHEMICAL-CHARACTERISTICS OF HUMAN AND KITTEN ODONTOCLASTS AND KITTEN OSTEOCLASTS - COMPARATIVE-STUDY USING WHOLE CELLS
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DOI：
10.1007/bf01004735
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发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
ADDISON, WC
中科院分区：
文献类型：
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作者：
ADDISON, WC
本文描述了破牙细胞和破骨细胞全细胞制剂中酶的组织化学显示方法。酶组织化学特征的人类和小猫破牙细胞从吸收乳牙和破骨细胞从小猫股骨干骺端进行了测定和比较。在所有3种类型的巨细胞中，酶谱、出现可检测反应产物的时间、反应强度和反应产物的定位相似。破牙细胞显然具有与破骨细胞相似的酶特性和代谢功能。种属差异似乎是轻微的，虽然NADP依赖性酶在人类中的活性低于小猫破牙细胞。破牙细胞和破骨细胞都富含与能量产生有关的酶，并且具有通常与分解代谢功能相关的酶的相当大的活性。代谢途径在利用琥珀酸、苹果酸、谷氨酸、乳酸和异柠檬酸方面发展良好，羟基丁酸和葡萄糖-6-磷酸，并且它们还具有磷酸酶、非特异性酯酶和亮氨酸萘酰胺酶。所证明的单个酶的酶反应产物的分布与这些细胞中大量线粒体和溶酶体样细胞器的存在一致。在中性和酸性pH值下，破牙细胞和破骨细胞中都有相当大的磷酸酶活性。
Methods for the histochemical demonstration of enzymes in whole cell preparations of odontoclasts and osteoclasts are described. Enzyme histochemical characteristics of human and kitten odontoclasts from resorbing primary teeth and of osteoclasts from kitten femur metaphyses were determined and compared. The enzyme profiles, times for the appearance of detectable reaction product, intensity of the reactions and localization of the reaction products were similar in all 3 types of giant cell. Odontoclasts apparently have enzyme properties and metabolic functions similar to those of osteoclasts. Species differences appear to be minor, although the NADP-dependent enzymes are less active in human than in kitten odontoclasts. Both odontoclasts and osteoclasts are rich in enzymes concerned with energy production and possess considerable activity of enzymes usually associated with catabolic functions. Metabolic pathways are well developed with respect to the utilization of succinic, malic, glutamic, lactic and isocitric acids, .beta.-hydroxybutyric acid and glucose-6-phosphate, and they also possess phosphatases, non-specific esterases and leucine naphthylamidase. The distribution of enzyme reaction products for the individual enzymes demonstrated is consistent with the presence in these cells of large numbers of mitochondria and lysosome-like organelles. Considerable phosphatase activity is demonstrable in both odontoclasts and osteoclasts at both neutral and acid pH.