Antigen-driven interactions with dendritic cells and expansion of Foxp3+ regulatory T cells occur in the absence of inflammatory signals
Antigen-driven interactions with dendritic cells and expansion of Foxp3+ regulatory T cells occur in the absence of inflammatory signals
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DOI：
10.4049/jimmunol.180.1.327
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发表时间：
2008-01-01
影响因子：
4.4
通讯作者：
Gross, David-Alexandre
中科院分区：
文献类型：
--
作者：
Chappert, Pascal;Leboeuf, Marylne;Gross, David-Alexandre
Foxp3(+) regulatory T cells (Tregs) play a pivotal role in the maintenance of peripheral T cell tolerance and are thought to interact with dendritic cells (DC) in secondary lymphoid organs. We analyzed here the in vivo requirements for selective expansion of Ag-specific Treg vs CD4(+)CD25(-) effector T cells and engagement of Ag-specific Treg-DC interactions in secondary lymphoid organs. Using i.v. Ag delivery in the absence of inflammation, we found that CD4(+)CD25(+)Foxp3(+) Tregs undergo vigorous expansion and accumulate whereas naive CD4(+)CD25(-)Foxp3(-) T cells undergo abortive activation. Quantifying directly the interactions between Tregs and CD11c(+) DC, we found that Tregs establish cognate contacts with endogenous CD11c(+) DC in spleen and lymph nodes at an early time point preceding their expansion. Importantly, we observed that as few as 10(3) Tregs selectively expanded by i.v. Ag injection are able to suppress B and T cell immune responses in mouse recipients challenged with the Ag. Our results demonstrate that Tregs are selectively mobilized by Ag recognition in the absence of inflammatory signals, and can induce thereafter potent tolerance to defined Ag targets.
