Pervanadate mediated an increased generation of inositol phosphates and tension in rat myometrium. Activation and phosphorylation of phospholipase C-gamma 1.
Pervanadate mediated an increased generation of inositol phosphates and tension in rat myometrium. Activation and phosphorylation of phospholipase C-gamma 1.
复制标题
过钒酸盐介导了大鼠子宫肌醇磷酸盐生成的增加和张力的增加。
作者：
B. Palmier;D. Leiber;S. Harbon
用原钒酸和H_2O_2混合形成的过钒酸盐刺激[~3H]肌醇标记的大鼠子宫肌条，可诱导[~3H]肌醇磷酸盐呈剂量依赖性积累。单独加入原钒酸盐或H_2O_2则不起作用。用两种酪氨酸激酶抑制剂，即金雀异黄素和酪氨酸蛋白47(100微米)对子宫肌层进行预处理，可将过钒刺激的肌醇磷酸生成减少50%。过钒酸盐诱导肌醇磷酸盐的形成按肌醇三磷酸、肌醇二磷酸和肌醇一磷酸的顺序依次形成。从三种肌醇磷酸酶的水平观察染料木素的抑制作用。过钒酸盐可引起子宫肌层收缩，其反应呈剂量依赖性。H_2O_2或原钒酸盐不起作用。金雀异黄素和酪磷蛋白47(100微米)可抑制过钒酸盐介导的收缩(50%)。使用抗磷酸酪氨酸抗体的Western印迹分析表明，在过钒酸盐刺激的子宫肌层的洗涤剂提取物中存在磷酸化蛋白。与100微米金雀异黄素或酪氨酸蛋白47预先孵育可减少酪氨酸磷酸化。磷脂酶C-Gamma1在子宫肌层提取物中被免疫检测到，并被鉴定为过钒酸盐处理后酪氨酸磷酸化的底物之一。结果表明，在子宫肌层中，蛋白酪氨酸激酶/磷酸酶活性控制着磷脂酶C-Gamma1的磷酸化和激活，有助于调节肌醇磷酸盐的生成和张力。
Stimulation of [3H]inositol-labeled rat myometrial strips with pervanadate, formed by mixing orthovanadate and H2O2, induced a dose-dependent accumulation of [3H]inositol phosphates. Orthovanadate or H2O2 added alone had no effect. Pretreatment of myometrium with two tyrosine kinase inhibitors, namely genistein and tyrphostin 47 (at 100 microM), reduced pervanadate-stimulated inositol phosphate formation by 50%. Pervanadate induced a time-sequential formation of inositol phosphates in the order inositol trisphosphate, inositol bisphosphate, and inositol monophosphate. The inhibitory effect of genistein was observed at the level of the three inositol phosphates. Pervanadate induced contraction of the myometrium; the response was dose-dependent. H2O2 or orthovanadate was without effect. Pervanadate-mediated contraction was inhibited (50%) by genistein and tyrphostin 47 (100 microM). Western blot analysis, using anti-phosphotyrosine antibodies, revealed that phosphorylated proteins were present in detergent extracts from pervanadate-stimulated myometrium. Tyrosine phosphorylation was reduced by a preincubation with 100 microM genistein or tyrphostin 47. Phospholipase C-gamma1 was immunodetected in myometrial extracts and was identified as one of the substrates subject to tyrosine phosphorylation following pervanadate treatment. The results demonstrate that, in myometrium, protein tyrosine kinase/phosphatase activities controlled both phosphorylation and activation of phospholipase C-gamma1, contributing to the modulation of the generation of inositol phosphates and tension.
影响因子：
4.8
作者：
C. Ku;Ansha Qian;Yeshao Wen;Khursheed Anwer;Barbara M. Sanborn
通讯作者：
C. Ku;Ansha Qian;Yeshao Wen;Khursheed Anwer;Barbara M. Sanborn
影响因子：
13.8
作者：
Hong Sun;N. Tonks
通讯作者：
Hong Sun;N. Tonks
表皮生长因子诱导的大鼠肝细胞中磷脂酶 C-gamma 1 的激活和易位至细胞骨架。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yang,LJ;Rhee,SG;Williamson,JR
通讯作者：
Williamson,JR