Temporal Relationship of Cyclic Nucleotide Levels and Calcium Exchange to Histamine-Induced Tension Development
Temporal Relationship of Cyclic Nucleotide Levels and Calcium Exchange to Histamine-Induced Tension Development
复制标题
环核苷酸水平和钙交换与组胺诱导的张力发展的时间关系
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
W. Speir
中科院分区：
文献类型：
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作者：
R. Kolbeck;W. Speir
本实验旨在研究离体培养的豚鼠气管平滑肌张力发展与组织cAMP、cGMP和Ca同位素水平变化的时间关系。剂量-反应研究进行了增加浓度的组胺在存在和不存在的H1受体阻滞剂使用10-5 M苯海拉明。随后在三种浓度的组胺存在下测定张力发展的时间过程，所述组胺显示引起50%（3 × 10-6 M）、85%（9 × 10-6 M）和100%（5 × 10-5 M）的最大收缩。在收缩开始后20秒、1分钟和6分钟测量组织环核苷酸和45 Ca水平。为了比较，卡巴胆碱的影响也进行了研究。我们的研究结果表明，在收缩变化的初始阶段，组织cAMP或cGMP水平没有可检测到的变化。与此相反，组织同位素钙摄取增加组胺诱导的收缩早期，并被H1拮抗剂。
Abstract The purpose of these experiments was to study the temporal relationship between tension development in incubated guinea pig tracheal smooth muscle and changes in tissue levels of cAMP and cGMP, and isotopic Ca. Dose–response studies were performed with increasing concentrations of histamine both in the absence and presence of H1 receptor blockade using 10-5 M diphenhydramine. The time course of tension development was subsequently determined in the presence of three concentrations of histamine shown to cause 50% (3 × 10-6 M), 85% (9 × 10-6 M), and 100% (5 × 10-5 M) of maximal contraction. Tissue cyclic nucleotide and 45Ca levels were measured 20 sec, 1 min, and 6 min after the onset of contraction. For comparison, the influence of carbachol was also studied. Our findings demonstrate that there were no detectable alterations in tissue cAMP or cGMP levels during the initial phases of contractile change. In contrast, tissue isotopic Ca uptake increased early in histamine-induced contraction and was blocked by the H1 antagonist.