A Panel of Angiogenic Factors for Early Bladder Cancer Detection: Enzyme Immunoassay and Western Blot
A Panel of Angiogenic Factors for Early Bladder Cancer Detection: Enzyme Immunoassay and Western Blot
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DOI：
10.1016/j.juro.2008.10.102
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发表时间：
2009-03-01
影响因子：
6.6
通讯作者：
El Ahmady, Omar
中科院分区：
文献类型：
--
作者：
Eissa, Sanaa;Swellam, Menha;El Ahmady, Omar
目的：血管生成受到大量促血管生成因子的严格调节，包括血管内皮生长因子、碱性成纤维细胞生长因子、肝细胞生长因子和血管生成素。我们适应和评估这些因素的测量，采用酶联免疫吸附试验和比较结果与Western印迹和排尿cytology.Materials和方法：本研究包括240例诊断为膀胱癌，108例良性膀胱病变和110名健康人作为对照。结果：血吸虫病血清学抗体检测、尿细胞学检查及尿血管生成因子检测的批内CV为10.3 ~ 12.3，批间CV为10 ~ 13.7。对于血管内皮生长因子、碱性成纤维细胞生长因子、血管生成素和肝细胞生长因子，向尿液池中添加的血管生成因子的回收率分别为98%-103%、97%-103%、98%-104%和97%-100%。与Westernblot法的符合率为97.5%。恶性肿瘤组尿血管生成标志物和尿细胞学检查的阳性率及水平均显著高于良性肿瘤组和健康对照组。膀胱鳞状细胞癌中碱性成纤维细胞生长因子明显升高。此外，碱性成纤维细胞生长因子和肝细胞生长因子与肿瘤分级显著相关。结论：应用酶联免疫吸附试验定量检测尿中血管生成因子是可靠的。碱性成纤维细胞生长因子和肝细胞生长因子的敏感性是上级的其他研究的标志物和细胞学在低级别和早期的情况下，表明他们的方便作为敏感的，非侵入性的诊断和筛查工具的膀胱癌。
Purpose: Angiogenesis is tightly regulated by a large number of pro-angiogenic factors, including vascular endothelial growth factor, basic fibroblast growth factor, hepatocyte growth factor and angiogenin. We adapted and evaluated the measurement of these factors using enzyme-linked immunosorbent assay and compared the results with Western blot and voided urine cytology.Materials and Methods: This study included 240 patients diagnosed with bladder carcinoma, 108 with benign bladder lesions and 110 healthy individuals who served as controls. All participants underwent serological schistosomiasis antibody assay in serum, urine cytology and estimation of angiogenic factors in voided urine.Results: Intra-assay and interassay CVs of the investigated markers were 10.3 to 12.3 and 10 to 13.7, respectively. The recovery rate of the added angiogenic factor to the urine pool was 98% to 103%, 97% to 103%, 98% to 104% and 97% to 100% for vascular endothelial growth factor, basic fibroblast growth factor, angiogenin and hepatocyte growth factor, respectively. The concordance rate with Western blot was 97.5%. The levels and positive rates of urinary angiogenic markers and urine cytology were significantly higher in the malignant group than in the benign and healthy groups. Basic fibroblast growth factor increased significantly in bladder squamous cell carcinoma cases. Moreover, basic fibroblast growth factor and hepatocyte growth factor significantly correlated with tumor grade. Angiogenic markers showed significant association with clinical stage.Conclusions: Quantitative measurement of urinary angiogenic factors in voided urine samples by enzyme-linked immunosorbent assay was reliable. The sensitivity of basic fibroblast growth factor and hepatocyte growth factor was superior to that of the other investigated markers and of cytology in low grade and early stage cases, suggesting their convenience as sensitive, noninvasive diagnostic and screening tools for bladder cancer.