Distribution and Diversity of Anaerobic Ammonium Oxidation (Anammox) Bacteria in the Sediment of a Eutrophic Freshwater Lake, Lake Kitaura, Japan
Distribution and Diversity of Anaerobic Ammonium Oxidation (Anammox) Bacteria in the Sediment of a Eutrophic Freshwater Lake, Lake Kitaura, Japan
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DOI：
10.1264/jsme2.me10184
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发表时间：
2011-09-01
影响因子：
2.2
通讯作者：
Suwa, Yuichi
中科院分区：
文献类型：
--
作者：
Yoshinaga, Ikuo;Amano, Teruki;Suwa, Yuichi
虽然N-2通过厌氧氨氧化（anammox）的排放是一个关键的过程中消除含氮化合物从水环境中，很少有信息是关于其意义和相关的微生物（anammox细菌）在富营养化淡水湖泊。在本研究中，在日本的富营养化湖泊，北浦湖，沉积物中的厌氧氨氧化细菌进行了研究，使用N-15-示踪技术，以测量其潜在的厌氧氨氧化活性。潜在的厌氧氨氧化活性定位于湖泊的北方区域，在那里的沉积物中存在稳定的NH 4+和NO3-的供应。这些结果表明，厌氧氨氧化细菌的贡献，从沉积物中的N-2在这个富营养化湖泊的总排放量是不可忽略的。此外，选择性PCR成功地扩增了厌氧氨氧化细菌相关（Brocadiales相关）的16 S rRNA基因从沉积物样品中观察到潜在的厌氧氨氧化活性。克隆文库中除Scalindua属外，均由不同的类群组成，其中Brocadia属的类群占优势，Kuenenia属的类群次之。然而，它们中的大多数都是新颖的，并且在系统发育上与已知的锦葵目物种不同。一种独特的厌氧氨氧化细菌种群栖息在北浦湖，并可能导致N-2的排放。
Although the emission of N-2 via anaerobic ammonium oxidation (anammox) is a key process in the elimination of nitrogenous compounds from aquatic environments, little information is available regarding its significance and the relevant microorganisms (anammox bacteria) in eutrophic freshwater lakes. In the present study, the anammox bacteria in the sediment of a eutrophic lake in Japan, Lake Kitaura, were examined using a N-15-tracer technique to measure their potential anammox activity. Potential anammox activity was localized to the northern region of the lake where a stable supply of both NH4+ and NO3- existed in the sediment. These results suggest the contribution of anammox bacteria to the total emission of N-2 from sediment in this eutrophic lake to not be negligible. Moreover, selective PCR successfully amplified anammox bacteria-related (Brocadiales-related) 16S rRNA genes from sediment samples in which potential anammox activity was observed. The clone libraries consisted of diverse phylotypes except the genus "Scalindua"-lineages, and the lineages of genus "Brocadia" were dominantly recovered, followed by the genus "Kuenenia"-lineages. Most of them, however, were novel and phylogenetically distinguishable from known Brocadiales species. A unique population of anammox bacteria inhabits and potentially contributes to the emission of N-2 from Lake Kitaura.