Proximo-distal specification in the wing disc of Drosophila by the nubbin gene
Proximo-distal specification in the wing disc of Drosophila by the nubbin gene
复制标题
DOI：
10.1073/pnas.94.21.11405
复制
发表时间：
1997-10-14
影响因子：
11.1
通讯作者：
GarciaBellido, A
中科院分区：
文献类型：
--
作者：
Cifuentes, FJ;GarciaBellido, A
Nubbin(Nub)基因突变的表型包括严重的翅膀尺寸缩小和模式变化，如远端元素向近端元素的转变。自从早期幼虫发育以来，Nub的表达仅限于翼盘中的翼囊细胞。这些效应也在遗传嵌合体中观察到，其中所有翼片区的细胞增殖都自发地减少，并在远端克隆中观察到向近端元件的转化。此外，位于翼片近端的无性系还会导致整个翅膀的非自主缩小。在根瘤突变背景下的细胞谱系实验表明，克隆不考虑前后或背腹边界，但选择基因从幼虫早期发育起就得到了正确的表达。块茎和块茎DPP基因组合的表型是协同的，DPP在块茎翅膀克隆中的过度表达不会导致周围野生型细胞的过度增殖。我们讨论了小节在机翼的远近轴线上的作用以及在隔室边界的形成中的作用。
Mutations in the nubbin (nub) gene have a phenotype consisting of a severe wing size reduction and pattern alterations, such as transformations of distal elements into proximal ones. nub expression is restricted to the wing pouch cells in wing discs since early larval development. These effects are also observed in genetic mosaics where cell proliferation is reduced in all wing blade regions autonomously, and transformation into proximal elements is observed in distal clones. Clones located in the proximal region of the wing blade cause in addition nonautonomous reduction of the whole wing. Cell lineage experiments in a nub mutant background show that clones respect neither the anterior-posterior nor the dorsal-ventral boundary but that the selector genes have been correctly expressed since early larval development. The phenotypes of nub el and nub dpp genetic combinations are synergistic and the overexpression of dpp in clones in nub wings does not result in overproliferation of the surrounding wild-type cells. We discuss the role of nub in the wing's proximo-distal axis and in the formation of compartment boundaries.