Immunity to rotavirus in T cell deficient
Immunity to rotavirus in T cell deficient
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DOI：
10.1006/viro.1997.8843
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发表时间：
1997-11-24
期刊：
影响因子：
3.7
通讯作者：
Greenberg, HB
中科院分区：
文献类型：
--
作者：
Franco, MA;Greenberg, HB
研究了轮状病毒在成年裸鼠(BALB/c背景)、α-β或γ-Delta和α-β/γ-Delta T细胞受体(TCR)基因敲除小鼠(C57BL/6和C57BL/6x129背景)以及SCID小鼠(C57BL/6背景)中的感染情况。Gamma Delta TCR/-清除了感染，就像对照组小鼠一样。与具有免疫能力的对照组小鼠相比，所有裸鼠(α-β、α-β/γ-TCR-/-小鼠)均清除了原发轮状病毒感染，并通过ELISA检测产生了轮状病毒特异性肠道IgA。用脾和板层细胞进行ELISPOT分析表明，免疫活性C57BL/6小鼠的病毒特异性肠道IgA应答与Gamma Delta TCR-/-小鼠相似，比杯TCR-/-和αβ/Gamma Delta TCR-/-小鼠高7-60倍。同样，裸鼠的反应是裸鼠的20倍。C57BL/6小鼠、γ-TCR-/-小鼠和裸鼠的肠道IgA抗体识别感染了轮状病毒VP6和VP4蛋白的重组杆状病毒感染的昆虫细胞，而α-βTCR-/-、α-β/γ-TCR-/-和裸鼠仅识别VP6。缺乏CD4(+)T细胞的免疫活性C57BL/6小鼠表现出与α-βTCR-/-小鼠相似的轮状病毒特异性肠道IgA水平，这表明这种T细胞非依赖的IgA反应在正常小鼠中存在。与之前发表的BALB/c SCID和RAG 2-/-(C57BL/6x129背景)小鼠都会慢性感染小鼠轮状病毒的结果相反，C57BL/6独居小鼠中40%的小鼠清除了原发轮状病毒感染。这些结果表明，T细胞非依赖性抗体反应和先天机制都有助于对小鼠轮状病毒的免疫，并表明伽玛-德尔塔T细胞不是有效清除小鼠原发轮状病毒感染所必需的。(C)1997年学术出版社。
Rotavirus infection was studied in adult nude mice (BALB/c background), alpha beta or gamma delta and alpha beta/gamma delta T cell receptor (TCR) knockout (-/-) mice (C57BL/6 and C57BL/6x129 backgrounds), and SCID mice (C57BL/6 background). The gamma delta TCR -/- cleared infection just like control mice. All of the nude mice, alpha beta, alpha beta/gamma delta TCR -/- mice cleared primary rotavirus infection, with a short delay, compared to immunocompetent control mice and developed a rotavirus-specific intestinal IgA measured by ELISA. Elispot analysis with spleen and lamina propia cells showed that the virus-specific intestinal IgA response in immunocompetent C57BL/6 mice was similar to the gamma delta TCR -/- mice and 7- to 60-fold higher than in the cup TCR -/- and alpha beta/gamma delta TCR -/- mice. Likewise, the response of nude +/- mice was 20 times greater than that of nude -/- littermates. While the intestinal IgA antibodies of C57BL/6 mice, gamma delta TCR -/- mice, and nude +/- mice recognized insect cells infected with recombinant baculovirus expressing rotavirus VP6 and VP4 proteins, those of the alpha beta TCR -/-, alpha beta/gamma delta TCR -/-, and nude -/- mice recognized only VP6. Immunocompetent C57BL/6 mice depleted of CD4(+) T cell developed similar levels of rotavirus-specific intestinal IgA as the alpha beta TCR -/- mice, suggesting that this T cell-independent IgA response is present in normal mice. in contrast to previously published results with BALB/c SCID and RAG 2 -/- (C57BL/6x129 background) mice, all of which become chronically infected with murine rotavirus, 40% of the C57BL/6 solo mice cleared primary rotavirus infection. These results suggest that both a T cell-independent antibody response and innate mechanisms can contribute to immunity to murine rotavirus and show that gamma delta T cells are not necessary for efficient clearance of primary rotavirus infection in mice. (C) 1997 Academic Press.