Microbacterium sorbitolivorans sp. nov., a novel member of Microbacteriaceae isolated from fermentation bed in pigpen.
Microbacterium sorbitolivorans sp. nov., a novel member of Microbacteriaceae isolated from fermentation bed in pigpen.
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DOI：
10.1099/ijsem.0.001556
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发表时间：
2016-12
影响因子：
2.8
通讯作者：
Yu-Chen Meng;Hong-Can Liu;Leilei Yang;YING-QIAN Kang;Yuguang Zhou;M. Cai
中科院分区：
文献类型：
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作者：
Yu-Chen Meng;Hong-Can Liu;Leilei Yang;YING-QIAN Kang;Yuguang Zhou;M. Cai
从福建省某猪舍发酵床中分离到两株细菌SZDIS-1- 1 T和GZDIS-1-1。细胞革兰氏染色阳性，厌氧性，短杆状，无鞭毛。与菌株SZDIS-1- 1 T的DNA-DNA杂交值分别为20.0±1.2、14.3±5.8和19.1± 1.6%。新分离物的DNA G+C含量为67.6- 71.1mol%，其主要脂肪酸为anteiso-C15：0、anteiso-C17：0和iso-C16：0。这些结果与微杆菌属的分类一致。细胞壁糖和特征氨基酸为鼠李糖、葡萄糖、半乳糖和鸟氨酸。主要的甲基萘醌是MK-11和MK-10。极性脂质由二磷脂酰甘油、磷脂酰甘油、糖脂、未知磷脂和未知脂质组成。这些特征构成了两个分离物的独特轮廓。因此，基于多相分类学特征，菌株SZDIS-1- 1 T和GZDIS-1-1代表微杆菌属的一个新种，建议将其命名为嗜橄榄微杆菌（Microbacteriumolivorans sp. nov.），模式菌株为菌株SZDIS-1- 1 T（=CGMCC 1.15228T=DSM 103422 T）。
Two bacterial strains SZDIS-1-1T and GZDIS-1-1 were isolated from fermentation bed of a pigpen in Fujian Province, China. Cells were Gram-stain-positive, facultatively anaerobic, short rods without flagellum. Their nearest phylogenetic neighbours were Microbacterium amylolyticum N5T (16S rRNA gene sequence similarity 98.2 %), Microbacterium indicum BBH6T (97.9 %) and Microbacterium gubbeenense DPC 5286T (97.8 %) with the DNA-DNA hybridization values to strain SZDIS-1-1T as 20.0±1.2, 14.3±5.8 and 19.1±1.6 %, respectively. The DNA G+C content of the new isolates was 67.6-71.1 mol% and anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0 were their predominant cellular fatty acids. These results were consistent with classification into the genus Microbacterium. However, cell-wall sugars and characteristic amino acid were rhamnose, glucose, galactose and ornithine. Major menaquinones were MK-11 and MK-10. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, glycolipids, unknown phospholipid and unknown lipids. These characteristics constructed a distinct profile of the two isolates. Therefore, based on polyphasic taxonomic characteristics, strains SZDIS-1-1T and GZDIS-1-1 represented a novel species of genus Microbacterium, for which the name Microbacterium sorbitolivorans sp. nov. is proposed, with strain SZDIS-1-1T (=CGMCC 1.15228T=DSM 103422T) as the type strain.