Indole-3-acetic acid production in Pseudomonas fluorescens HP72 and its association with suppression of creeping bentgrass brown patch
Indole-3-acetic acid production in Pseudomonas fluorescens HP72 and its association with suppression of creeping bentgrass brown patch
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DOI：
10.1007/s00284-002-3968-2
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发表时间：
2003-08-01
影响因子：
2.6
通讯作者：
Oyaizu, H
中科院分区：
文献类型：
--
作者：
Suzuki, S;He, YX;Oyaizu, H
荧光假单胞菌 HP72 可抑制翦股颖褐斑病，产生多种次生代谢物：2,4-二乙酰间苯三酚 (2,4-DAPG)、HCN、铁载体和吲哚-3-乙酸 (IAA)。在本研究中，研究了 HP72 菌株中 IAA 的生物合成。经过多次重复传代培养，分离出自发IAA低产突变体HP72LI。菌株 HP72LI 的 IAA 产量低是由于色氨酸侧链氧化酶 (TSO) 活性低。菌株 HP72 在翦股颖根上的定殖引起根生长减少，而菌株 HP72LI 不引起这种生长减少。菌株HP72在翦股颖根上的定殖能力高于菌株HP72LI。然而，就生防能力而言，两种菌株均未检测到显着差异。菌株 HP72 产生的 IAA 可能在短根系统的构建中发挥作用并利用根定殖，但对荧光假单胞菌 HP72 的生物防治特性没有贡献。
Pseudomonas fluorescens HP72, which suppresses the brown patch disease on bentgrass, produces several secondary metabolites, 2,4-diacetylphloroglucinol (2,4-DAPG), HCN, siderophore, and indole-3-acetic acid (IAA). In this study, IAA biosynthesis in strain HP72 was investigated. After several repeated subcultures, the spontaneous IAA low-producing mutant HP72LI was isolated. The IAA low production of the strain HP72LI was due to the low tryptophan side chain oxidase (TSO) activity. Colonization of strain HP72 on the bentgrass root induced root growth reduction, while strain HP72LI did not induce such growth reduction. The colonization ability of strain HP72 on the bentgrass root is higher than that of strain HP72LI. However, as for biocontrol ability, a significant difference in both strains was not detected. IAA production by strain HP72 may play a role in the construction of short root systems and take advantage of root colonization, but does not contribute to the biocontrol properties of P. fluorescens HP72.