Packaging the replicon RNA of the Far-Eastern subtype of tick-borne encephalitis virus into single-round infectious particles: development of a heterologous gene delivery system.
Packaging the replicon RNA of the Far-Eastern subtype of tick-borne encephalitis virus into single-round infectious particles: development of a heterologous gene delivery system.
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DOI：
10.1016/j.vaccine.2005.03.004
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发表时间：
2005-06
期刊：
影响因子：
5.5
通讯作者：
K. Yoshii;D. Hayasaka;A. Goto;Kazue Kawakami;H. Kariwa;I. Takashima
中科院分区：
文献类型：
--
作者：
K. Yoshii;D. Hayasaka;A. Goto;Kazue Kawakami;H. Kariwa;I. Takashima
通过提供反式病毒结构蛋白，将蜱传脑炎 (TBE) 病毒（远东亚型）的亚基因组复制子包装成感染性颗粒。 TBE 复制子 RNA 和表达结构蛋白的质粒的顺序转染导致含有 TBE 复制子 RNA 的感染性颗粒的分泌。分泌的颗粒具有单轮传染性，可被TBE病毒中和抗体抑制。这些颗粒的物理结构与感染性病毒粒子的物理结构几乎相同，并且包装的复制子RNA未显示与病毒结构蛋白的mRNA发生重组。此外，通过包装带有插入的 GFP 和 Neo 基因的 TBE 复制子 RNA，成功传递和表达异源基因。该复制子包装系统可能是TBE病毒基因组包装机制的分子研究和疫苗递送系统开发的有用工具。
The sub-genomic replicon of tick-borne encephalitis (TBE) virus (Far-Eastern subtype) was packaged into infectious particles by providing the viral structural proteins in trans. Sequential transfection of TBE replicon RNA and a plasmid that expressed the structural proteins led to the secretion of infectious particles that contained TBE replicon RNA. The secreted particles had single-round infectivity, which was inhibited by TBE virus-neutralizing antibody. The physical structure of the particles was almost identical to that of infectious virions, and the packaged replicon RNA showed no recombination with the mRNAs of the viral structural proteins. Furthermore, heterologous genes were successfully delivered and expressed by packaging TBE replicon RNA with inserted GFP and Neo genes. This replicon packaging system may be a useful tool for the molecular study of the TBE virus genome packaging mechanism, and for the development of vaccine delivery systems.