APPLICATION OF INVIVO ELECTROCHEMISTRY TO THE MEASUREMENT OF CHANGES IN DOPAMINE RELEASE DURING INTRACRANIAL SELF-STIMULATION
APPLICATION OF INVIVO ELECTROCHEMISTRY TO THE MEASUREMENT OF CHANGES IN DOPAMINE RELEASE DURING INTRACRANIAL SELF-STIMULATION
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DOI：
10.1016/0165-0270(90)90050-p
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发表时间：
1990-09-01
影响因子：
3
通讯作者：
PHILLIPS, AG
中科院分区：
文献类型：
--
作者：
BLAHA, CD;PHILLIPS, AG
在同侧腹侧被盖区（VTA）沿着植入双极刺激电极的同时，将硬脂酸修饰的石墨糊记录电极急性或慢性植入丘脑腹侧被盖区（VTA）。计时电流法被用来监测电化学信号的变化，可能对应于多巴胺（DA）的氧化在实验者给予的刺激（EAS）和颅内自我刺激（ICS）。应用EAS刺激电极在腹侧被盖区产生增加的电化学信号在麻醉和清醒的准备。这两种影响的大小增加作为电流强度的函数。ICS的启动也伴随着电化学信号的立即增加。速率-强度实验揭示了ICS速率和电化学信号随着电流强度的连续增加或减少而相应增加。在随后的实验中，腹膜内注射DA摄取阻断剂诺米芬新和GBR-12909产生显着增加的计时电流信号的振幅，这对应于药物诱导的增加压杆速率。去甲肾上腺素能吸收阻滞剂地昔帕明对ICS速率或氧化电流均无显着影响。这些数据表明，ICS的腹侧被盖区可能会产生并发增加多巴胺神经传递的延髓核。药理学研究与腹侧被盖区电极部位脑刺激奖励的多巴胺能底物一致。
Stearate-modified graphite paste recording electrodes were acutely or chronically implanted into the nucleus accumbens along with bipolar stimulating electrodes in the ipsilateral ventral tegmental area (VTA). Chronoamperometry was used to monitor changes in electrochemical signals that may correspond to the oxidation of dopamine (DA) during experimenter-administered stimulation (EAS) and intracranial self-stimulation (ICS). Application of EAS to stimulating electrodes in the VTA produced increases in the electrochemical signal in both the anesthetized and conscious preparation. The magnitude of both effects increased as a function of current intensity. Initiation of ICS was also accompanied by an immediate increase in the electrochemical signal. Rate-intensity experiments revealed a corresponding increase in both the ICS rates and the electrochemical signal with successive increases or decreases in current intensity. In subsequent experiments, intraperitoneal injections of DA uptake blockers nomifensine and GBR-12909 produced significant increases in the amplitude of the chronoamperometric signal which corresponded to drug-induced increases in bar press rates. The noradrenergic uptake blocker desipramine had no significant effect on either ICS rates or oxidation current. These data indicate that ICS of the VTA may produce concurrent increases in DA neurotransmission in the nucleus accumbens. The pharmacological studies are consistent with a dopaminergic substrate of brain stimulation reward at electrode sites in the VTA.