Low nanogram detection of nucleotides using fast atom bombardment-mass spectrometry.
Low nanogram detection of nucleotides using fast atom bombardment-mass spectrometry.
复制标题
使用快原子轰击质谱法检测低纳克核苷酸。
DOI：
10.1016/0003-2697(89)90363-1
复制
发表时间：
1989
影响因子：
2.9
通讯作者：
Townsend,LB
中科院分区：
文献类型：
--
作者：
Weng,QM;Hammargren,WM;Slowikowski,D;Schram,KH;Borysko,KZ;Wotring,LL;Townsend,LB
The effect of trimethylsilyl (TMS) derivatization on detection limits of mononucleotides in fast atom bombardment-mass spectrometry (FAB-MS) was examined. FAB-MS methods were developed to optimize sensitivity using adenosine 5′-monophosphate as a model compound and then applied to reference standards of two clinically important nucleotides: tricyclic nucleoside-5′-monophosphate (TCNMP) and 5-fluoro-2′-deoxyuridine-5′-monophosphate (FdUMP). The detection limit for the TMS derivative of TCNMP was 2.5 – 5 ng/μl and less than 2.5 ng/μl for FdUMP as its TMS derivative. This is greater than two orders of magnitude more sensitive than the FAB-MS analysis of the corresponding free compounds. These low detection limits for the TMS derivatives were obtained using a narrow scan range, signal averaging, detection in the negative ion mode, and 3-nitrobenzyl alcohol as the matrix. Hydrolysis of one or more of the labile TMS groups did occur, with the extent of hydrolysis being greatest in the more protic matrices.
