Establishment of human-embryonic-stem-cell line from mosaic trisomy 9 embryo
Establishment of human-embryonic-stem-cell line from mosaic trisomy 9 embryo
复制标题
从嵌合9三体胚胎建立人胚胎干细胞系
DOI：
10.1016/j.tjog.2015.08.003
复制
发表时间：
2015-10-01
影响因子：
2.1
通讯作者：
Liu, Jiayin
中科院分区：
文献类型：
--
作者：
Huang, Boxian;Jiang, Chunyan;Liu, Jiayin
目的：人类胚胎干细胞(HESC)系是由植入前遗传学诊断后获得的染色体或遗传学异常胚胎获得的，在遗传疾病研究中具有重要价值。材料和方法：本研究通过分离、培养和传代马赛克9三体胚胎的内细胞团，建立了一种新的hESC系--临床生殖医学中心8(CCRM8)。结果：染色体核型分析表明，该hESC系为整倍体，共46条染色体。未分化的hESCs具有长期增殖能力，并表达OCT4、TRA-1-60和TRA-1-81等典型标志物。体外类胚体(EB)的形成、分化和体内畸胎瘤的产生证实了hESC系的多能性。本研究首次详细报道了1个中国人嵌合体9三体胚胎hESC系的特征和分化。结论：染色体异常胚胎可以产生正常的hESC系，这将有助于基因功能和胚胎发育的研究。版权所有(C)2015，台湾妇产科协会。爱思唯尔台湾有限责任公司出版。版权所有。
Objective: Human-embryonic-stem-cell (hESC) lines derived from chromosomally or genetically abnormal embryos obtained following preimplantation genetic diagnosis are valuable in investigating genetic disorders.Materials and methods: In this study, a new hESC line, Center of Clinical Reproductive Medicine 8 (CCRM8) was established by isolation, culture, and passaging of the inner cell mass of mosaic trisomy 9 embryos.Results: A karyotype analysis showed that the hESC line possessed a euploid (46 chromosomes). The undifferentiated hESCs exhibited long-term proliferation capacity and expressed typical markers of OCT4, TRA-1-60, and TRA-1-81. In vitro embryoid-body (EB) formation, differentiation, and in vivo teratoma production confirmed the pluripotency of the hESC line. The data represented here are the first detailed report on the characterization and differentiation of one Chinese hESC line generated from mosaic trisomy 9 embryos.Conclusion: Our study showed that chromosomally aberrant embryos could generate a normal hESC line, which would be useful in investigating gene function and embryo development. Copyright (C) 2015, Taiwan Association of Obstetrics & Gynecology. Published by Elsevier Taiwan LLC. All rights reserved.