Cajal body number and nucleolar size correlate with the cell body mass in human sensory ganglia neurons
Cajal body number and nucleolar size correlate with the cell body mass in human sensory ganglia neurons
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DOI：
10.1016/j.jsb.2006.12.008
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发表时间：
2007-06-01
影响因子：
3
通讯作者：
Lafarga, Miguel
中科院分区：
文献类型：
--
作者：
Berciano, Maria T.;Novell, Mariona;Lafarga, Miguel
本文研究了无神经系统疾病患者尸检组织中分离的人背根神经节（DRG）神经元中核仁和Cajal小体（CB）的细胞大小依赖性组织。用抗原纤蛋白抗体对核仁进行定量分析，结果表明所有神经元只有一个核仁。然而，核仁体积和每个核仁的纤维中心的数量显着增加作为细胞体大小的函数。卷曲蛋白的免疫染色证明了DRG神经元中存在大量CB（在大尺寸神经元中多达20个）。每个神经元的CB数与细胞体体积呈正相关。光镜和电镜免疫细胞化学分析显示，在DRG神经元的CB中的coilin，snRNP，SMN和fibrillarin的浓度。CB常与核仁、染色质活性区和PML小体相关，但与端粒无关。我们的研究结果支持这样的观点，即核仁体积和纤维中心和CB的数量取决于细胞体质量，这是一个与哺乳动物神经元的转录和突触活动密切相关的参数。此外，不寻常的大量CB可以促进RNA加工组分从CB转移到RNA加工的核仁和核质位点。(c)2007年爱思唯尔公司All rights reserved.
This paper studies the cell size-dependent organization of the nucleolus and Cajal bodies (CBs) in dissociated human dorsal root ganglia (DRG) neurons from autopsy tissue samples of patients without neurological disease. The quantitative analysis of nucleoli with an anti-fibrillarin antibody showed that all neurons have only one nucleolus. However, the nucleolar volume and the number of fibrillar centers per nucleolus significantly increase as a function of cell body size. Immunostaining for coilin demonstrated the presence of numerous CBs in DRG neurons (up to 20 in large size neurons). The number of CBs per neuron correlated positively with the cell body volume. Light and electron microscopy immunocytochemical analysis revealed the concentration of coilin, snRNPs, SMN and fibrillarin in CBs of DRG neurons. CBs were frequently associated with the nucleolus, active chromatin domains and PML bodies, but not with telomeres. Our results support the view that the nucleolar volume and number of both fibrillar centers and CBs depend on the cell body mass, a parameter closely related to transcriptional and synaptic activity in mammalian neurons. Moreover, the unusual large number of CBs could facilitate the transfer of RNA processing components from CBs to nucleolar and nucleoplasmic sites of RNA processing. (c) 2007 Elsevier Inc. All rights reserved.