Dynamic acetylation of lysine-4-trimethylated histone H3 and H3 variant biology in a simple multicellular eukaryote.
Dynamic acetylation of lysine-4-trimethylated histone H3 and H3 variant biology in a simple multicellular eukaryote.
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DOI：
10.1093/nar/gks367
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发表时间：
2012-08
影响因子：
14.9
通讯作者：
Mahadevan LC
中科院分区：
文献类型：
--
作者：
Hsu DW;Chubb JR;Muramoto T;Pears CJ;Mahadevan LC
所有赖氨酸-4-三甲基化组蛋白H3的动态乙酰化是后生真核生物中参与即刻早期基因诱导的复杂现象。高等真核生物表达三个密切相关的H3变异体的重复拷贝，无法进行遗传分析。我们证明了这些现象在有三个单拷贝H3变异基因的网柄网柄菌中是保守的。虽然动态乙酰化针对两个K4-三甲基化的H3变体，但K9-乙酰化优先针对一个。在缺乏Set1甲基转移酶和任何可检测到的K4-三甲基化的细胞中，动态乙酰化丢失，证明了两者之间的直接联系。表达突变的H3变异体的基因替换揭示了不同变异体上K4-三甲基化之间的一种新的相互作用。只表达一种变体的细胞在生长和紫外线诱导基因的诱导方面存在缺陷，证明了变体表达的功能重要性。这些研究证实，针对H3K4me3的动态乙酰化发生在进化的早期，并揭示了在简单的多细胞真核生物中利用组蛋白变体的高度特异性。
Dynamic acetylation of all lysine-4-trimethylated histone H3 is a complex phenomenon involved in Immediate-early gene induction in metazoan eukaryotes. Higher eukaryotes express repeated copies of three closely related H3 variants, inaccessible to genetic analysis. We demonstrate conservation of these phenomena in Dictyostelium which has three single-copy H3 variant genes. Although dynamic acetylation is targeted to two H3 variants which are K4-trimethylated, K9-acetylation is preferentially targeted to one. In cells lacking Set1 methyltransferase and any detectable K4-trimethylation, dynamic acetylation is lost demonstrating a direct link between the two. Gene replacement to express mutated H3 variants reveals a novel interaction between K4-trimethylation on different variants. Cells expressing only one variant show defects in growth, and in induction of a UV-inducible gene, demonstrating the functional importance of variant expression. These studies confirm that dynamic acetylation targeted to H3K4me3 arose early in evolution and reveal a very high level of specificity of histone variant utilization in a simple multicellular eukaryote.
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影响因子：
2.7
作者：
Chubb, JR;Bloomfield, G;Singer, RH
通讯作者：
Singer, RH
影响因子：
14.9
作者：
Kuhlmann M;Borisova BE;Kaller M;Larsson P;Stach D;Na J;Eichinger L;Lyko F;Ambros V;Söderbom F;Hammann C;Nellen W
通讯作者：
Nellen W
影响因子：
9.8
作者：
Hazzalin, CA;Mahadevan, LC
通讯作者：
Mahadevan, LC
影响因子：
14.9
作者：
Dubin, Manu;Fuchs, Joerg;Nellen, Wolfgang
通讯作者：
Nellen, Wolfgang
影响因子：
4.8
作者：
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通讯作者：
Hunt, Donald F.