Primary central nervous system lymphoma secretes monocyte chemoattractant protein 1
Primary central nervous system lymphoma secretes monocyte chemoattractant protein 1
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DOI：
10.1007/s00795-006-0350-7
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发表时间：
2007-03-01
影响因子：
1.8
通讯作者：
Kubota, Toshihiko
中科院分区：
文献类型：
--
作者：
Kitai, Ryuhei;Ishisaka, Kazuyo;Kubota, Toshihiko
原发性中枢神经系统淋巴瘤（PCNSL）多数为弥漫性大b细胞淋巴瘤。组织学上，在PCNSL组织中发现反应性T淋巴细胞和单组织细胞。为了阐明细胞浸润的机制，我们采用免疫组织化学染色、激光共聚焦显微镜双染色和Western blot分析19例PCNSL活检标本中单核细胞化学引诱蛋白（MCP-1）的存在。免疫组织化学观察到MCP-1在所有PCNSL中的表达。Western blot分析显示，PCNSL中MCP-1的浓度与转移性脑肿瘤中一样高。正常脑组织中未检测到MCP-1。共聚焦激光显微镜下发现，MCP-1信号存在于具有CD20 （b细胞标志物）的细胞中。我们得出结论，淋巴瘤细胞产生MCP-1，这是一种参与PCNSL发病机制的额外细胞因子。
The majority of primary central nervous system lymphomas (PCNSL) are diffuse large B-cell lymphomas. Histologically, reactive T lymphocytes and monohistiocytic cells are found within PCNSL tissue. To clarify the mechanisms of the cellular infiltration, the presence of monocyte chemoattractant protein (MCP-1) was investigated in biopsy samples of 19 cases of PCNSL by means of immunohistochemical staining, double staining with a confocal laser microscope, and Western blot analysis. MCP-1 expression was observed in all PCNSL immunohistochemically. Western blot analysis showed that the concentration of MCP-1 in PCNSL was as high as that in a metastatic brain tumor. In normal brain tissue, MCP-1 was not detected. Confocal laser microscope revealed MCP-1 signals were present in the cells with CD20, a B-cell marker. We concluded that lymphoma cells produced MCP-1, which is an additional cytokine involved in the pathogenesis of PCNSL.