Acute low-dose melamine affects hippocampal synaptic plasticity and behavior in rats
Acute low-dose melamine affects hippocampal synaptic plasticity and behavior in rats
复制标题
急性低剂量三聚氰胺影响大鼠海马突触可塑性和行为
DOI：
10.1016/j.toxlet.2012.08.010
复制
发表时间：
2012-10-02
影响因子：
3.5
通讯作者：
Mao, Rong-Rong
中科院分区：
文献类型：
--
作者：
Yang, Yuan;Xiong, Gui-Jing;Mao, Rong-Rong
受三聚氰胺污染的食品可能对人体健康造成危害。然而，三聚氰胺的神经毒性尚未得到充分评估。本研究旨在探讨急性低剂量三聚氰胺暴露对大鼠海马突触可塑性和行为的影响。我们发现，浴应用50-500微克/毫升三聚氰胺减少基础突触传递的Schaffer侧支-CA 1通路的海马脑片从出生后（P）10-14天的浓度依赖性的方式;此外，这种减少在传输有关的突触前功能的减少，如增加的成对脉冲易化率。与P10-14和P21-28大鼠相比，2-3月龄的大鼠对500 μ g/ml三聚氰胺对基础突触传递的影响不太敏感。三聚氰胺（50 μ g/ml）显着损害长时程增强（LTP），而不影响长时程抑郁（LTD），在P10-14和2-3个月大的大鼠。行为测试前1 h经口给予三聚氰胺（5和25 mg/kg）可显著降低2-3月龄大鼠强迫游泳测试的不动时间，但对P21-28和2-3月龄大鼠旷场测试的自发活动无影响。我们的研究结果揭示了急性低剂量三聚氰胺引起的海马突触可塑性和行为的神经毒性的一些方面。(c)2012爱思唯尔爱尔兰有限公司保留所有权利。
Foods contaminated with melamine potentially cause risk to human health. However, the neurotoxicity of melamine has not been adequately assessed. Here, we aimed to examine the effects of acute low-dose exposure to melamine on hippocampal synaptic plasticity and behaviors in rats. We found that bath application of 50-500 mu g/ml melamine decreased basal synaptic transmission in the Schaffer collateral-CA1 pathway of hippocampal slices from postnatal days (P) 10-14 rats in a concentration-dependent manner; furthermore, this decrease in transmission was related to the reduction of presynaptic function as indicated by the increased paired-pulse facilitation ratio. Rats at 2-3 months old were less vulnerable to the effects of 500 mu g/ml melamine on basal synaptic transmission when compared with P10-14 and P21-28 rats. Melamine (50 mu g/ml) significantly impaired long-term potentiation (LTP), without affecting long-term depression (LTD), in both P10-14 and 2-3 month-old rats. Oral treatment with melamine (5 and 25 mg/kg) 1 h before behavioral tests significantly decreased the immobility time of the forced swim test in 2-3 month-old rats and had no effect on locomotor activity in the open field test in both P21-28 and 2-3 month-old rats. Our findings reveal some of the aspects of neurotoxicity induced by acute low-dose of melamine in hippocampal synaptic plasticity and behavior. (c) 2012 Elsevier Ireland Ltd. All rights reserved.