ANTIGENICALLY DISTINCT POPULATIONS OF NEISSERIA-GONORRHOEAE - ISOLATION AND CHARACTERIZATION OF RESPONSIBLE DETERMINANTS
ANTIGENICALLY DISTINCT POPULATIONS OF NEISSERIA-GONORRHOEAE - ISOLATION AND CHARACTERIZATION OF RESPONSIBLE DETERMINANTS
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DOI：
10.1093/infdis/130.6.619
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发表时间：
1974-01-01
影响因子：
6.4
通讯作者：
APICELLA, MA
中科院分区：
文献类型：
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作者：
APICELLA, MA
在随机选择的10株淋球菌菌株中鉴定出两个抗原上不同的淋病奈瑟菌群，暂定为gc1和Gc2。通过deae -纤维素层析从这些菌株碱消化的酚水提取物中分离出导致这种分化的抗原决定因子。丙烯酰胺凝胶的免疫扩散、免疫电泳和电聚焦研究表明，分离的抗原制剂基本上只含有一种成分。免疫学分析表明，这些抗原除了各自的人群特异性决定因素外，还有一个共同的核心决定因素。免疫化学和物理化学研究表明，gc1和gc2抗原是具有非常相似的电荷和大小特征的酸性多糖。
Two antigenically distinct populations ofNeisseria gonorrhoeae, tentatively designated Gc1and Gc2, have been identified in 10 randomly selected gonococcal strains. The antigenic determinants responsible for this differentiation have been isolated by DEAE-cellulose chromatography from the alkali-digested phenolwater extract of these strains. Immunodiffusion, immunoelectrophoresis, and electrofocusing studies in acrylamide gel indicate that isolated antigen preparations contain essentially one component. Immunological analysis reveals that these antigens share a core determinant in addition to their respective population-specific determinants. Immunochemical and physicochemical studies demonstrate that the Gc1and Gc2antigens are acidic polysaccharides with very similar charge and size characteristics.