Metabolic pathways regulated by γ-aminobutyric acid (GABA) contributing to heat tolerance in creeping bentgrass (Agrostis stolonifera).
Metabolic pathways regulated by γ-aminobutyric acid (GABA) contributing to heat tolerance in creeping bentgrass (Agrostis stolonifera).
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DOI：
10.1038/srep30338
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发表时间：
2016-07-26
影响因子：
4.6
通讯作者：
Huang B
中科院分区：
文献类型：
--
作者：
Li Z;Yu J;Peng Y;Huang B
γ-氨基丁酸是一种参与多种代谢过程的非蛋白氨基酸。本研究的目的是通过生理分析来检验增加GABA是否可以提高冷季匍匐弯草的耐热性，并通过代谢谱分析来确定GABA调节的主要代谢途径。采用0.5 mM GABA或水对植物进行预处理，然后在受控生长室内进行非胁迫条件（21/19°C）或热胁迫（35/30°C）处理35 d。生长和生理分析表明，外源GABA显著提高了匍匐草的耐热性。代谢谱分析发现，外源应用GABA导致氨基酸（谷氨酸、天冬氨酸、丙氨酸、苏氨酸、丝氨酸和缬氨酸）、有机酸（乌头酸、苹果酸、琥珀酸、草酸和苏酸）、糖（蔗糖、果糖、葡萄糖、半乳糖和麦芽糖）和糖醇（甘露醇和肌醇）的积累增加。这些结果表明，GABA诱导的匍生弯草的耐热性可能涉及光合作用和抗坏血酸-谷胱甘肽循环的增强，渗透调节的维持以及GABA分流的增加。GABA分流的增加可能是在长期热应激过程中为呼吸代谢的三羧酸循环提供中间产物，从而维持代谢稳态。
γ-Aminobutyric acid is a non-protein amino acid involved in various metabolic processes. The objectives of this study were to examine whether increased GABA could improve heat tolerance in cool-season creeping bentgrass through physiological analysis, and to determine major metabolic pathways regulated by GABA through metabolic profiling. Plants were pretreated with 0.5 mM GABA or water before exposed to non-stressed condition (21/19 °C) or heat stress (35/30 °C) in controlled growth chambers for 35 d. The growth and physiological analysis demonstrated that exogenous GABA application significantly improved heat tolerance of creeping bentgrass. Metabolic profiling found that exogenous application of GABA led to increases in accumulations of amino acids (glutamic acid, aspartic acid, alanine, threonine, serine, and valine), organic acids (aconitic acid, malic acid, succinic acid, oxalic acid, and threonic acid), sugars (sucrose, fructose, glucose, galactose, and maltose), and sugar alcohols (mannitol and myo-inositol). These findings suggest that GABA-induced heat tolerance in creeping bentgrass could involve the enhancement of photosynthesis and ascorbate-glutathione cycle, the maintenance of osmotic adjustment, and the increase in GABA shunt. The increased GABA shunt could be the supply of intermediates to feed the tricarboxylic acid cycle of respiration metabolism during a long-term heat stress, thereby maintaining metabolic homeostasis.