Transcriptome analysis of genes involved in defence response in Polyporus umbellatus with Armillaria mellea infection.
Transcriptome analysis of genes involved in defence response in Polyporus umbellatus with Armillaria mellea infection.
复制标题
蜜环菌感染猪苓防御反应相关基因的转录组分析
DOI：
10.1038/srep16075
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发表时间：
2015-11-03
影响因子：
4.6
通讯作者：
Guo SX
中科院分区：
文献类型：
--
作者：
Liu MM;Xing YM;Zhang DW;Guo SX
猪苓是蜜环菌的共生菌，对蜜环菌的侵染也表现出明显的防御反应。目前还没有基因组学资源数据库来研究猪苓感染胁迫的分子机制。因此，我们用A.使用Illumina测序技术检测Mellea感染。干净读段的组装产生120，576个转录物，包括38，444个单基因。此外，我们在感染治疗后进行了基因表达谱分析。结果表明，在对照组和感染组之间的基因表达谱的显着差异。在两组之间总共鉴定了10933个基因。基于差异表达的基因，基因本体注释分析显示了许多防御相关的类别。与此同时，《京都基因与基因组百科全书》的通路分析揭示了一些重要的通路。此外，13个推定的基因，参与防御反应，从定量实时PCR的表达模式是一致的，其转录丰度变化所确定的RNA-seq。测序的基因覆盖了侧耳转录组的相当大的比例，表达结果可能有助于加强对该真菌防御蜜环菌入侵的防御反应的认识。
Polyporus umbellatus, a species symbiotic with Armillaria mellea and it also exhibits substantial defence response to Armillaria mellea infection. There are no genomics resources databases for understanding the molecular mechanism underlying the infection stress of P. umbellatus. Therefore, we performed a large-scale transcriptome sequencing of this fungus with A. mellea infection using Illumina sequencing technology. The assembly of the clean reads resulted in 120,576 transcripts, including 38,444 unigenes. Additionally, we performed a gene expression profiling analysis upon infection treatment. The results indicated significant differences in the gene expression profiles between the control and the infection group. In total, 10933 genes were identified between the two groups. Based on the differentially expressed genes, a Gene Ontology annotation analysis showed many defence-relevant categories. Meanwhile, the Kyoto Encyclopedia of Genes and Genomes pathway analysis uncovered some important pathways. Furthermore, the expression patterns of 13 putative genes that are involved in defence response resulting from quantitative real-time PCR were consistent with their transcript abundance changes as identified by RNA-seq. The sequenced genes covered a considerable proportion of the P. umbellatus transcriptome and the expression results may be useful to strengthen the knowledge on the defence response of this fungus defend against Armillaria mellea invasion.