RETRACTION OF F PILI
RETRACTION OF F PILI
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DOI：
10.1128/jb.117.3.1306-1311.1974
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发表时间：
1974-01-01
影响因子：
3.2
通讯作者：
FIVESTAY.P
中科院分区：
文献类型：
--
作者：
NOVOTNY, CP;FIVESTAY.P
用电子显微镜和血清阻断力研究了在10−2M NaCN存在下，F菌毛在大肠杆菌细胞上的消失情况。从细胞中消失的菌毛在培养上清液中没有出现游离菌毛，说明菌毛已经回缩到细胞内。去除NaCN后约3分钟，以正常速率产生新菌毛。F菌毛抗体或R17噬菌体吸附在菌毛两侧和低于24℃的温度下可防止回缩。菌毛的消失伴随着细胞对R17噬菌体吸附能力的丧失，以及抑制R17噬菌体感染和交配的F菌毛抗体类型的丧失。吸附M13噬菌体的能力和抑制M13噬菌体感染的F菌毛抗体类型不受影响。这表明，缩回的菌毛尖端裸露。
The disappearance of F pili onEscherichia colicells in the presence of 10−2M NaCN was studied by electron microscopy and serum-blocking power. The pili which disappeared from the cell did not appear as free pili in the culture medium, suggesting that the pili had retracted into the cell. New pili were produced at a normal rate approximately 3 min after NaCN was removed. The adsorption of either F pili antibody or R17 bacteriophage to the sides of pili and temperatures below 24 C prevented retraction. The disappearance of pili was accompanied by a loss in the ability of cells to adsorb R17 phage and the type of F pili antibody that inhibits R17 phage infection and mating. The ability to adsorb M13 phage and the type of F pili antibody that inhibits M13 phage infection was not affected. This suggests that the tips of retracted pili are exposed.