Modes of propagation of Ca(2+)-induced Ca2+ release in bullfrog sympathetic ganglion cells.
Modes of propagation of Ca(2+)-induced Ca2+ release in bullfrog sympathetic ganglion cells.
复制标题
牛蛙交感神经节细胞中 Ca(2) 诱导的 Ca2 释放的传播模式。
作者：
S. Hua;C. Liu;F. Lu;M. Nohmi;K. Kuba
How depolarization-induced Ca2+ entry or caffeine activates Ca(2+)-induced Ca2+ release (CICR) in the cytoplasm and nucleoplasm was studied by recording intracellular Ca2+ ([Ca2+]i) with a confocal microscope in cultured bullfrog sympathetic ganglion cells. The amplitude and propagation speed of voltage pulse-induced rises in [Ca2+]i were greater in the submembrane (< 5 microns depth) region than in the core region, and delayed and smaller, but significant, in the nucleus. Ryanodine and dantrolene reduced the rises in [Ca2+]i in both the cytoplasm and nucleus. A rapid application of high K+ solution induced global rises in [Ca2+]i in both the cytoplasm and nucleoplasm, which were decreased by dantrolene. Caffeine produced a slow, small rise in [Ca2+]i which grew into a global, regenerative rise both in the cytoplasm and nucleoplasm with some inward gradient in the cytoplasm. Each of the high [Ca2+]i phases during caffeine-induced [Ca2+]i oscillation began in the submembrane region, while low [Ca2+]i phases started in the core region. These results suggest that CICR activated by Ca2+ entry or caffeine occurs predominantly in the submembrane region causing an inwardly spreading Ca2+ wave or [Ca2+]i oscillations, and that the nuclear envelope can cause CICR in the nucleoplasm, which is delayed due to Ca2+ diffusion barrier at the nuclear pores.
DOI：
10.1016/s0021-9258(18)35875-7
发表时间：
1992-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
E. Buck;I. Zimanyi;J. Abramson;I. Pessah
通讯作者：
E. Buck;I. Zimanyi;J. Abramson;I. Pessah
影响因子：
2.5
作者：
K. Moore;A. Cohen;J. Kao;D. Weinreich
通讯作者：
K. Moore;A. Cohen;J. Kao;D. Weinreich
影响因子：
--
作者：
ROUSSEAU, E;SMITH, JS;MEISSNER, G
通讯作者：
MEISSNER, G