CYTOKINE MESSENGER-RNA EXPRESSION OF BONE-MARROW STROMAL CELLS FROM PATIENTS WITH APLASTIC-ANEMIA AND MYELODYSPLASTIC SYNDROME
CYTOKINE MESSENGER-RNA EXPRESSION OF BONE-MARROW STROMAL CELLS FROM PATIENTS WITH APLASTIC-ANEMIA AND MYELODYSPLASTIC SYNDROME
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DOI：
10.1111/j.1365-2141.1993.tb03208.x
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发表时间：
1993-12-01
影响因子：
6.5
通讯作者：
NIITSU, Y
中科院分区：
文献类型：
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作者：
HIRAYAMA, Y;KOHGO, Y;NIITSU, Y
研究了9例正常人、8例再生障碍性贫血（AA）患者和7例骨髓增生异常综合征（MDS）患者骨髓基质细胞中细胞因子粒细胞集落刺激因子（G-CSF）、白细胞介素-1 β (il -1 β)、IL-6、IL-8和干细胞因子的mRNA表达。内皮细胞、巨噬细胞、成纤维细胞样细胞和脂肪细胞在基质细胞中的比例在正常志愿者和患者之间没有差异。逆转录聚合酶链反应检测细胞因子mRNA表达水平。在所有正常志愿者和大多数患者的细胞中都发生了自发表达，并且通过LPS刺激增强了自发表达。在LPS刺激下，AA患者G-CSF和il -1 β mRNA的平均表达量显著高于正常志愿者，但有1例患者il -1 β、IL-6和IL-8表达较低，对LPS无反应。lps诱导的2例MDS患者IL-6、IL-8表达明显高于正常水平。15例、10例和4例患者培养上清液中自发和lps诱导的G-CSF、IL-6和IL-8蛋白浓度与mRNA表达呈良好相关性。相关系数分别为0.92、0.78和0.91。综上所述，有少数患者的病因表现为AA中基质细胞因子表达减少，但大多数AA和MDS患者的细胞因子mRNA表达正常或高水平。
We studied mRNA expression of the cytokine granulocyte-colony stimulating factor (G-CSF), interleukin-1beta (IL-1beta), IL-6, IL-8 and stem cell factor of stromal cells derived from bone marrows of nine normal volunteers, eight patients with aplastic anaemia (AA) and seven patients with myelodysplastic syndrome (MDS). The proportion of endothelial cells, macrophages, fibroblast-like cells and adipocytes in stromal cells showed no differences between normal volunteers and the patients. Levels of cytokine mRNA expression were determined by reverse transcription-polymerase chain reaction. Spontaneous expression occurred and this was augmented by LPS stimulation in cells of all the normal volunteers and in most patients.When stimulated by LPS, the mean G-CSF and IL-1beta mRNA expressions in patients with AA were significant higher than normal volunteers, but there was one patient showing lower IL-1beta, IL-6 and IL-8 expression with no response to LPS. LPS-induced IL-6 and IL-8 expression of two patients with MDS were significantly higher than normal.The spontaneous and LPS-induced protein concentration of G-CSF, IL-6 and IL-8 in culture supernatants from 15, 10 and four patients, correlated well with the mRNA expression. The correlation coefficients were 0.92, 0.78 and 0.91, respectively.In conclusion, there were a few patients whose aetiology appeared to be reduction of stromal cytokine expression in AA, but most patients with AA and MDS expressed normal or high levels of cytokine mRNA.