Sex differences in L-type calcium current after chronic ethanol consumption in rats.
Sex differences in L-type calcium current after chronic ethanol consumption in rats.
复制标题
大鼠慢性乙醇消耗后 L 型钙电流的性别差异。
DOI：
10.1016/s0041-008x(03)00125-x
复制
发表时间：
2003
影响因子：
3.8
通讯作者：
Liu,ShiJ
中科院分区：
文献类型：
--
作者：
Kennedy,RichardH;Liu,ShiJ
慢性酒精消耗会导致进行性心脏收缩功能障碍，对大鼠的研究表明，这种酒精性心肌疾病在男性中比女性更明显。与乙醇诱导的心脏毒性相关的细胞变化在很大程度上尚未确定;然而，L型钙通道电流（ICa，L）可能受到影响。使用全细胞膜片钳技术，本研究检查了ICa，林成年心室肌细胞分离的男性和女性的P-大鼠饮用水（对照）或25%的乙醇/水混合物14个月。与对照雄性大鼠相比，从乙醇消耗中分离的心肌细胞中，ICa，L的峰值幅度和最大电导分别增加了32%和26%。与此相反，没有观察到的ICa，L的幅度或电导的差异时，从控制和乙醇消耗的女性分离的心肌细胞进行比较。乙醇处理对两种性别的动力学ICa、Linactivation或稳态激活和失活均无显著影响。总之，男性而不是女性心肌细胞响应于慢性乙醇消耗增加的ICa，L，这可能是一种补偿性反应的抑制收缩。
Chronic ethanol consumption elicits a progressive cardiac contractile dysfunction, and studies in rats suggest that this alcoholic heart muscle disease is more pronounced in males than females. Cellular changes associated with the ethanol-induced cardiotoxicity remain largely undefined; however, it is possible that L-type Ca2+channel current (ICa,L) is affected. Using whole-cell patch-clamp techniques, this study examined ICa,Lin adult ventricular myocytes isolated from male and female P-rats that had consumed drinking water (controls) or a 25% ethanol/water mixture for 14 months. The peak amplitude and maximum conductance of ICa,Lwere 32 and 26% greater, respectively, in cardiomyocytes isolated from ethanol-consuming compared to control male rats. In contrast, no differences in the amplitude or conductance of ICa,Lwere observed when comparing myocytes isolated from control and ethanol-consuming females. Ethanol treatment had no significant effects on the kinetics ICa,Linactivation or on steady-state activation and inactivation in either gender. In conclusion, male but not female cardiomyocytes respond to chronic ethanol consumption with an increased ICa,Lthat may represent a compensatory response to the depressed contractility.