CLONING OF CDNAS AND THE MOLECULAR EVOLUTION OF A BOVINE MHC CLASS-II DRA GENE
CLONING OF CDNAS AND THE MOLECULAR EVOLUTION OF A BOVINE MHC CLASS-II DRA GENE
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DOI：
10.1006/bbrc.1994.2444
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发表时间：
1994-10-14
影响因子：
3.1
通讯作者：
ASAHINA, M
中科院分区：
文献类型：
--
作者：
AIDA, Y;KOHDA, C;ASAHINA, M
分离并表征了编码牛主要组织相容性复合物 (MKC) II 类抗原 DRA 链的两个重叠 cDNA 克隆。全长cDNA克隆MR1编码253个氨基酸的初级翻译产物，其中24个氨基酸被推断为信号肽，229个氨基酸形成成熟多肽。从该克隆推导的氨基酸序列在大小和结构上与其他物种的 II A 类分子相似，但在 α2 结构域以及羊和马 DRA 分子中没有发现与人、小鼠、大鼠和猪蛋白中的 N 连接糖基化相似的潜在共有位点。对几个物种之间 II A 类基因编码的氨基酸序列进行比较，并根据这些数据构建树状图，将 DRA 基因和 DQA/DYA 基因置于系统发育树的两个不同分支上，其中牛 DRA 和绵羊 DRA 在 DRA 分支上最相似。 (C) 1994 年学术出版社
Two overlapping cDNA clones coding for bovine major histocompatibility complex (MKC) class II antigen DRA chain were isolated and characterized. The full-length cDNA clone, MR1, encoded a primary translated product of 253 amino acids, 24 of which were deduced to be a signal peptide and 229 which formed a mature polypeptide. The amino acid sequences deduced from this clone resembled those of class II A molecules from other species in both size and structure, but no potential consensus site of N-linked glycosylation comparable to those in the human, mouse, rat and swine proteins was found in the alpha 2 domain, as well as ovine and equine DRA molecules. Comparison of amino acid sequences encoded by class II A genes among several species and a dendrogram constructed from these data places the DRA gene and the DQA/DYA genes on two distinct branches of a phylogenetic tree, with bovine DRA and ovine DRA being most similar on the DRA branch. (C) 1994 Academic Press, Inc.