Human dendritic cell 1 and dendritic cell 2 subsets express FcεF-RI:: Correlation with serum IgE and allergic asthma
Human dendritic cell 1 and dendritic cell 2 subsets express FcεF-RI:: Correlation with serum IgE and allergic asthma
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DOI：
10.1016/j.jaci.2003.09.011
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发表时间：
2003-12-01
影响因子：
14.2
通讯作者：
Prussin, C
中科院分区：
文献类型：
--
作者：
Foster, B;Metcalfe, DD;Prussin, C
背景资料：1型树突状细胞（DC 1）表达高亲和力IgE受体（FcepsilonFRI）;然而，DC对FcepsilonRI表达的调控尚不清楚。尚未证实Fc ε RI的2型DC（DC 2）表达。目的：我们假设DC 2细胞也表达FcepsilonRI，并且DC 1和DC 2亚群的FcepsilonRI表达与血清IgE和过敏性哮喘疾病状态相关。研究方法：为了验证这些假设，我们通过使用流式细胞术定量外周血前体DC 1（pDC 1）和pDC 2亚群的Fc ε RI α链表达。结果：pDC 1和pDC 2亚群及扁桃体组织DC均表达Fc受体RI。嗜碱性粒细胞、pDC 1和pDC 2亚群的相对Fc ε RI表达分别为12：6.5：1。在两个pDC亚群中，过敏性哮喘受试者中FcepsilonRI的表达显著高于非特异性对照受试者。pDC 1和pDC 2的表达与血清IgE浓度高度相关。pDC 1、pDC 2和嗜碱性粒细胞亚群表现出与血清IgE变化相关的Fc ε RI表达增加幅度相似。结论：DC 1和DC 2亚型均有FcepsilonRI的表达。此外，这些细胞的FcepsilonRI表达与血清IgE和嗜碱性粒细胞FcepsilonRI表达高度相关，并且在过敏性哮喘受试者中更高。这些数据支持这样的概念，即直接靶向Fc ε RI表达的新型治疗方法将影响过敏原特异性免疫应答的致敏和效应阶段。
Background: Type 1 dendritic cells (DC1) express the high-affinity IgE receptor (FcepsilonFRI); however, the regulation of FcepsilonRI expression by DCs is not well understood. Type 2 DC (DC2) expression of FcepsilonRI has not been demonstrated. Objective: We hypothesized that DC2 cells also express FcepsilonRI and that expression of FcepsilonRI by the DC1 and DC2 subsets correlates with serum IgE and allergic asthma disease status. Methods: To test these hypotheses, we quantitated FcepsilonRI a chain expression by the peripheral blood precursor DC1 (pDC1) and pDC2 subsets by using flow cytometry. Results: FcepsilonRI was expressed by the pDC1 and pDC2 subsets, as well as tissue DCs from tonsils. Relative FcepsilonRI expression by basophil, pDC1, and pDC2 subsets was 12:6.5:1, respectively. In both pDC subsets, FcepsilonRI expression was significantly greater in allergic asthmatic subjects than in nonatopic control subjects. pDC1 and pDC2 expression of FcepsilonRI was highly correlated to serum IgE concentration. The pDC1, pDC2, and basophil subsets demonstrated a similar magnitude of increase in FcepsilonRI expression relative to changes in serum IgE. Conclusions: FcepsilonRI expression is characteristic of both the DC1 and DC2 subsets. Furthermore, FcepsilonRI expression by these cells is highly correlated to serum IgE and to basophil FcepsilonRI expression and is greater in subjects with allergic asthma. These data support the concept that novel therapeutic approaches directly targeted at FcepsilonRI expression would affect both the sensitization and the effector phases of the allergen-specific immune response.