Expression of inflammatory mediators in the otitis media induced by Helicobacter pylori antigen in mice
Expression of inflammatory mediators in the otitis media induced by Helicobacter pylori antigen in mice
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DOI：
10.1111/j.1365-2249.2008.03740.x
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发表时间：
2008-10
影响因子：
4.6
通讯作者：
S. Kariya;M. Okano;K. Fukushima;S. Nomiya;Y. Kataoka;Rie Nomiya;H. Akagi;K. Nishizaki
中科院分区：
文献类型：
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作者：
S. Kariya;M. Okano;K. Fukushima;S. Nomiya;Y. Kataoka;Rie Nomiya;H. Akagi;K. Nishizaki
幽门螺杆菌是一种革兰氏阴性菌，被认为是胃炎、消化性溃疡和胃癌等胃病的关键因素之一。最近的研究表明H. pylori和消化道外疾病，以及H.已经报道了中耳和上呼吸道中的幽门螺杆菌。然而，H.幽门螺杆菌在中耳疾病中的作用尚不清楚。本研究表明，H. pylori全细胞蛋白直接诱导小鼠中耳上皮细胞中巨噬细胞移动抑制因子、巨噬细胞炎性蛋白2、白细胞介素1β和肿瘤坏死因子α，接种H. pylori全细胞蛋白。此外，经鼓室注射巨噬细胞移动抑制因子可上调中耳巨噬细胞炎性蛋白2的表达。这些发现表明H. pylori感染可引起中耳上皮免疫性炎症，H.幽门螺杆菌可能在中耳炎中起重要作用。
Helicobacter pylori is a Gram‐negative bacterium that is recognized as one of the key factors in gastric diseases such as gastritis, peptic ulcer and gastric cancer. Recent studies have shown relationships between H. pylori and extra‐digestive diseases, and the presence of H. pylori in the middle ear and upper respiratory tract has been reported. However, the role of H. pylori in middle ear disease remains unclear. The present study demonstrated that H. pylori whole‐cell protein directly induces macrophage migration inhibitory factor, macrophage inflammatory protein 2, interleukin 1β and tumor necrosis factor α in middle ear epithelium in mice, and severe proliferation of inflammatory cells was observed in middle ear cavity inoculated with H. pylori whole‐cell protein. In addition, trans‐tympanic injection of macrophage migration inhibitory factor up‐regulated expression of macrophage inflammatory protein 2 in the middle ear. These findings indicate that H. pylori infection causes immunological inflammation in middle ear epithelium, and H. pylori may play a significant role in otitis media.