PMS1T, producing phased small-interfering RNAs, regulates photoperiod-sensitive male sterility in rice
PMS1T, producing phased small-interfering RNAs, regulates photoperiod-sensitive male sterility in rice
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PMS1T，产生阶段性小干扰RNA，调节水稻光周期敏感雄性不育
DOI：
10.1073/pnas.1619159114
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发表时间：
2016-12-27
影响因子：
11.1
通讯作者：
Zhang, Qifa
中科院分区：
文献类型：
--
作者：
Fan, Yourong;Yang, Jiangyi;Zhang, Qifa
定相小干扰RNA（Phased small-interfering RNA，phasiRNA）是一类特殊的小RNA，由前体RNA的转录产物以21-或24-nt的间隔产生。虽然phasiRNA已经在一系列生物体中发现，但它们在植物中的生物学功能尚未被发现。在这里，我们表明，phasiRNAs产生的光周期敏感的雄性不育1（Pms 1）基因座与水稻，种质开始的两系杂交水稻育种的光周期敏感的雄性不育（PSMS）。Pms 1基因座编码一个在幼穗中优先表达的长非编码RNAPMS 1 T，通过miR 2118靶向PMS 1 T产生21-nt的phasiRNA，在长日照条件下优先在PSMS系中积累。PMS 1 T中miR 2118识别位点附近的单核苷酸多态性对生育力变化至关重要，可能导致phasiRNA的差异积累。这一结果表明了phasiRNA在水稻生殖发育中的可能作用，证明了这类RNA在生物过程中作为调节剂的潜在重要性。
Phased small-interfering RNAs (phasiRNAs) are a special class of small RNAs, which are generated in 21- or 24-nt intervals from transcripts of precursor RNAs. Although phasiRNAs have been found in a range of organisms, their biological functions in plants have yet to be uncovered. Here we show that phasiRNAs generated by the photopheriod-sensetive genic male sterility 1 (Pms1) locus were associated with photoperiod-sensitive male sterility (PSMS) in rice, a germplasm that started the two-line hybrid rice breeding. ThePms1locus encodes a long-noncoding RNAPMS1Tthat was preferentially expressed in young panicles.PMS1Twas targeted by miR2118 to produce 21-nt phasiRNAs that preferentially accumulated in the PSMS line under long-day conditions. A single nucleotide polymorphism inPMS1Tnearby the miR2118 recognition site was critical for fertility change, likely leading to differential accumulation of the phasiRNAs. This result suggested possible roles of phasiRNAs in reproductive development of rice, demonstrating the potential importance of this RNA class as regulators in biological processes.