Rugosibacter aromaticivorans gen. nov., sp nov., a bacterium within the family Rhodocyclaceae, isolated from contaminated soil, capable of degrading aromatic compounds
Rugosibacter aromaticivorans gen. nov., sp nov., a bacterium within the family Rhodocyclaceae, isolated from contaminated soil, capable of degrading aromatic compounds
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DOI：
10.1099/ijsem.0.001622
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发表时间：
2017-02-01
影响因子：
2.8
通讯作者：
Singleton, David R.
中科院分区：
文献类型：
--
作者：
Corteselli, Elizabeth M.;Aitken, Michael D.;Singleton, David R.
从美国北卡罗来纳州夏洛特市的一家人工制气厂旧址的多环芳烃(PAH)污染土壤中分离到一株细菌Ca6(T)，该菌株与贝塔变形杆菌纲的红环菌科有亲缘关系。它的16S rRNA基因序列与全球分布的环境序列高度相似，包括通过稳定同位素探测证明生长在多环芳烃菲和芘上的那些先前被命名为Py Group 1的序列。与亲缘关系最近的亲缘关系是食氢硫杆菌株sk43H(T)(16S rRNA基因序列同源性为93.6%)。除了有限数量的有机酸外，Ca6(T)还能够生长在单芳烃苯和甲苯以及氮芳烃咔唑上，作为唯一的碳和能源来源。在多环芳烃、菲和芘上的生长也得到证实。在中温、中性pH和低盐度条件下，最适生长条件为好氧。存在的主要脂肪酸包括总和特征3(C-16：1 omega 7c或C-16：1 omega 6c)和C-16：0。测序结果表明，该单条染色体的DNA G+C含量为55.14%。由于其独特的遗传和生理特性，Ca6(T)菌株被认为是红树科一个新的属和种的成员，命名为Rugosibacter aromaticiorans gen。Nov.，sp.11月是提议的。该种的模式株为Ca6(T)(=ATCC TSD-59(T)=DSM 103039(T))。
A bacterial strain designated Ca6(T) was isolated from polycyclic aromatic hydrocarbon (PAH)-contaminated soil from the site of a former manufactured gas plant in Charlotte, NC, USA, and linked phylogenetically to the family Rhodocyclaceae of the class Betaproteobacteria. Its 16S rRNA gene sequence was highly similar to globally distributed environmental sequences, including those previously designated 'Pyrene Group 1' demonstrated to grow on the PAHs phenanthrene and pyrene by stable-isotope probing. The most closely related described relative was Sulfuritalea hydrogenivorans strain sk43H(T) (93.6% 16S rRNA gene sequence identity). In addition to a limited number of organic acids, Ca6(T) was capable of growth on the monoaromatic compounds benzene and toluene, and the azaarene carbazole, as sole sources of carbon and energy. Growth on the PAHs phenanthrene and pyrene was also confirmed. Optimal growth was observed aerobically under mesophilic temperature, neutral pH and low salinity conditions. Major fatty acids present included summed feature 3 (C-16:1 omega 7c or C-16:1 omega 6c) and C-16 :0. The DNA G+C content of the single chromosome was 55.14 mol% as determined by complete genome sequencing. Due to its distinct genetic and physiological properties, strain Ca6(T) is proposed as a member of a novel genus and species within the family Rhodocyclaceae, for which the name Rugosibacter aromaticivorans gen. nov., sp. nov. is proposed. The type strain of the species is Ca6(T) (= ATCC TSD-59(T) =DSM 103039(T)).