Conservation of dishevelled structure and function between flies and mice: Isolation and characterization of Dvl2
Conservation of dishevelled structure and function between flies and mice: Isolation and characterization of Dvl2
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DOI：
10.1016/s0925-4773(96)00549-7
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发表时间：
1996-08-01
影响因子：
2.6
通讯作者：
Sussman, DJ
中科院分区：
文献类型：
--
作者：
Klingensmith, J;Yang, Y;Sussman, DJ
果蝇的体节极性基因dishevelled（dsh）是胚胎体节和成虫成虫盘图案形成所必需的。dsh编码Wingless信号转导途径中最早作用和最特异的已知组分，Wingless是小鼠中与Wnt 1同源的细胞外信号。我们先前已经描述了Dvl1小鼠dsh同源物的分离和表征。我们在这里报告的第二个小鼠dsh同源物，Dvl2，它映射到11号染色体的分离。Dvl2氨基酸序列与Dvl1的dsh序列同样相关，但Dvl2与非洲爪蟾同源物Xdsh最相似。然而，与其他脊椎动物dsh同系物不同。与其他基因一样，Dvl2在大多数胚胎发生中普遍表达，并在许多成体器官中表达。我们已经开发了一种测定果蝇胚胎中dsh功能的方法，并表明Dvl2可以部分挽救缺乏dsh的胚胎的分割缺陷。因此，Dvl2编码dsh的哺乳动物同源物，其可以抑制无翅信号。
The segment polarity gene dishevelled (dsh) of Drosophila is required for pattern formation of the embryonic segments and the adult imaginal discs. dsh encodes the earliest-acting and most specific known component of the signal transduction pathway of Wingless, an extracellular signal homologous to Wnt1 in mice. We have previously described the isolation and characterization of the Dvl1 mouse dsh homolog. We report here the isolation of a second mouse dsh homolog, Dvl2, which maps to chromosome 11. The Dvl2 amino acid sequence is equally related to the dsh sequence as is that of Dvl1, but Dvl2 is most similar to the Xenopus homolog Xdsh. However, unlike the other vertebrate dsh homologs. Like the other genes, Dvl2 is ubiquitously expressed throughout most of embryogenesis and is expressed in many adult organs. We have developed an assay for dsh function in fly embryos, and show that Dvl2 can partially rescue the segmentation defects of embryos devoid of dsh. Thus, Dvl2 encodes a mammalian homolog of dsh which can transduce the Wingless signal.